Literature DB >> 23545637

Crystallization and X-ray structure determination of a thermoalkalophilic lipase from Geobacillus SBS-4S.

Muhammad Tayyab1, Naeem Rashid, Clement Angkawidjaja, Shigenori Kanaya, Muhammad Akhtar.   

Abstract

A thermoalkalophilic lipase (LIPSBS) from the newly isolated Geobacillus strain SBS-4S which hydrolyzes a wide range of fatty acids has been characterized. In the present study, the crystallization of purified LIPSBS using the sitting-drop vapour-diffusion method and its X-ray diffraction studies are described. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 55.13, b = 71.75, c = 126.26 Å. The structure was determined at 1.6 Å resolution by the molecular-replacement method using the lipase from G. stearothermophilus L1 as a model.

Entities:  

Keywords:  Geobacillus SBS-4S; crystal structure; lipases; molecular replacement; thermoalkalophilic

Mesh：

Substances：

Year:  2013        PMID： 23545637      PMCID： PMC3614156          DOI： 10.1107/S1744309113004910

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

1.  Novel zinc-binding center and a temperature switch in the Bacillus stearothermophilus L1 lipase.

Authors:  Seong-Tae Jeong; Hyung-Kwoun Kim; Seung-Jun Kim; Seung-Wook Chi; Jae-Gu Pan; Tae-Kwang Oh; Seong-Eon Ryu
Journal:  J Biol Chem       Date:  2002-02-21       Impact factor: 5.157

2.  Structure validation by Calpha geometry: phi,psi and Cbeta deviation.

Authors:  Simon C Lovell; Ian W Davis; W Bryan Arendall; Paul I W de Bakker; J Michael Word; Michael G Prisant; Jane S Richardson; David C Richardson
Journal:  Proteins       Date:  2003-02-15

3.  Isolation and identification of lipase producing thermophilic Geobacillus sp. SBS-4S: cloning and characterization of the lipase.

Authors:  Muhammad Tayyab; Naeem Rashid; Muhammad Akhtar
Journal:  J Biosci Bioeng       Date:  2010-12-24       Impact factor: 2.894

4.  Coot: model-building tools for molecular graphics.

Authors:  Paul Emsley; Kevin Cowtan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-11-26

5.  Molecular replacement with MOLREP.

Authors:  Alexei Vagin; Alexei Teplyakov
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2009-12-21

6.  Crystallography & NMR system: A new software suite for macromolecular structure determination.

Authors:  A T Brünger; P D Adams; G M Clore; W L DeLano; P Gros; R W Grosse-Kunstleve; J S Jiang; J Kuszewski; M Nilges; N S Pannu; R J Read; L M Rice; T Simonson; G L Warren
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1998-09-01

Review 7.  Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.

Authors:  K E Jaeger; B W Dijkstra; M T Reetz
Journal:  Annu Rev Microbiol       Date:  1999       Impact factor: 15.500

8.  Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements.

Authors:  César Carrasco-López; César Godoy; Blanca de Las Rivas; Gloria Fernández-Lorente; José M Palomo; José M Guisán; Roberto Fernández-Lafuente; Martín Martínez-Ripoll; Juan A Hermoso
Journal:  J Biol Chem       Date:  2008-12-03       Impact factor: 5.157

9.  REFMAC5 for the refinement of macromolecular crystal structures.

Authors:  Garib N Murshudov; Pavol Skubák; Andrey A Lebedev; Navraj S Pannu; Roberto A Steiner; Robert A Nicholls; Martyn D Winn; Fei Long; Alexei A Vagin
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18

10.  Overview of the CCP4 suite and current developments.

Authors:  Martyn D Winn; Charles C Ballard; Kevin D Cowtan; Eleanor J Dodson; Paul Emsley; Phil R Evans; Ronan M Keegan; Eugene B Krissinel; Andrew G W Leslie; Airlie McCoy; Stuart J McNicholas; Garib N Murshudov; Navraj S Pannu; Elizabeth A Potterton; Harold R Powell; Randy J Read; Alexei Vagin; Keith S Wilson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18
  10 in total

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