OBJECTIVE: To determine the involvement of Toll-like receptor (TLR) 2, TLR9, and TLR2+9 in renal ischemia/reperfusion (I/R) injury. METHODS: Mice with gene knock-out of TLR2, TLR9, and TLR2+9 underwent renal I/R injury. Tubular damage, expression of proinflammatory cytokines and chemokines, and kidney dysfunction were evaluated on different days after reperfusion. RESULTS: Mice deficient in TLR2, but not TLR9, were protected from renal I/R injury with less tubular damage, decreased cytokine production, and lower serum creatinine and urea levels than wild-type mice. TLR2+9 double-deficiency did not have an additional effect on tissue injury compared with TLR2 deficiency alone. CONCLUSION: These results suggest that activation of TLR2 signaling contributes to the pathogenesis of renal I/R injury, whereas TLR9 may be redundant for the development of this injury.
OBJECTIVE: To determine the involvement of Toll-like receptor (TLR) 2, TLR9, and TLR2+9 in renal ischemia/reperfusion (I/R) injury. METHODS:Mice with gene knock-out of TLR2, TLR9, and TLR2+9 underwent renal I/R injury. Tubular damage, expression of proinflammatory cytokines and chemokines, and kidney dysfunction were evaluated on different days after reperfusion. RESULTS:Mice deficient in TLR2, but not TLR9, were protected from renal I/R injury with less tubular damage, decreased cytokine production, and lower serum creatinine and urea levels than wild-type mice. TLR2+9double-deficiency did not have an additional effect on tissue injury compared with TLR2 deficiency alone. CONCLUSION: These results suggest that activation of TLR2 signaling contributes to the pathogenesis of renal I/R injury, whereas TLR9 may be redundant for the development of this injury.
Authors: Sang Jun Han; Ryan M Williams; Vivette D'Agati; Edgar A Jaimes; Daniel A Heller; H Thomas Lee Journal: Kidney Int Date: 2020-02-22 Impact factor: 10.612
Authors: Maria Giovanna De Leo; Leopoldo Staiano; Mariella Vicinanza; Alessandro Luciani; Annamaria Carissimo; Margherita Mutarelli; Antonella Di Campli; Elena Polishchuk; Giuseppe Di Tullio; Valentina Morra; Elena Levtchenko; Francesca Oltrabella; Tobias Starborg; Michele Santoro; Diego Di Bernardo; Olivier Devuyst; Martin Lowe; Diego L Medina; Andrea Ballabio; Maria Antonietta De Matteis Journal: Nat Cell Biol Date: 2016-07-11 Impact factor: 28.824
Authors: Pieter J Bakker; Angelique M Scantlebery; Loes M Butter; Nike Claessen; Gwendoline J D Teske; Tom van der Poll; Sandrine Florquin; Jaklien C Leemans Journal: PLoS One Date: 2015-09-11 Impact factor: 3.240