| Literature DB >> 23539897 |
Chunyan Lu1, Runjun Yang, Binglei Shen, Hassan Osman, Yonghong Zhang, Shouqing Yan, Liying Zhang, Zhihui Zhao.
Abstract
Diacylglyceroltransferase-1 (DGAT1) expresses in nearly all tissues, including the mammary gland. Mice lacking DGAT1 exhibit decreased triglyceride content in mammary tissue, and are resistant to diet-induced obesity and diabetes mellitus. Thus, DGAT1 has received considerable attention. In the present study, the function of DGAT1 was examined by liposome mediated RNA interference (RNAi) to knockdown the expression of endogenous DGAT1 expression in bovine mammary epithelial cells (BMEC) and the changes of the biological functions of cells were analyzed. The mRNA and protein levels, intracellular triglyceride (TG) content, and total protein of BMECs were analyzed by real-time PCR, Western blot, TG kit, and ultraviolet spectrophotometer, respectively, before and after RNAi treatment. The results indicated that knockdown of DGAT1 expression significantly reduced TG content in BMECs. This study further confirmed the importance of DGAT1 in triglyceride synthesis in bovine mammary tissue.Entities:
Mesh：
Substances：
Year: 2012 PMID： 23539897 PMCID： PMC6043834 DOI： 10.3727/105221613x13571653093123
Source DB: PubMed Journal: Gene Expr ISSN： 1052-2166