OBJECTIVES: This study examined the phenomenon of heteroresistance in Mycobacterium tuberculosis clinical isolates obtained from retreated patients in Beijing, China between 2006 and 2011. METHODS: The iPLEX Gold assay platform was used to determine the prevalence of heteroresistance to injectable second-line drugs (amikacin, kanamycin and capreomycin) in resistant isolates. RESULTS: Heteroresistance was identified in 10.9% of 220 phenotypic amikacin-resistant isolates. CONCLUSIONS: Heteroresistance was related mainly to the short duration and repeated use of amikacin and capreomycin during retreatment. These findings further our understanding of the evolution of resistance to injectable drugs used for tuberculosis treatment and help guide the rational use of injectable drugs during therapy.
OBJECTIVES: This study examined the phenomenon of heteroresistance in Mycobacterium tuberculosisclinical isolates obtained from retreated patients in Beijing, China between 2006 and 2011. METHODS: The iPLEX Gold assay platform was used to determine the prevalence of heteroresistance to injectable second-line drugs (amikacin, kanamycin and capreomycin) in resistant isolates. RESULTS: Heteroresistance was identified in 10.9% of 220 phenotypic amikacin-resistant isolates. CONCLUSIONS: Heteroresistance was related mainly to the short duration and repeated use of amikacin and capreomycin during retreatment. These findings further our understanding of the evolution of resistance to injectable drugs used for tuberculosis treatment and help guide the rational use of injectable drugs during therapy.
Authors: Amanda C Brown; Josephine M Bryant; Katja Einer-Jensen; Jolyon Holdstock; Darren T Houniet; Jacqueline Z M Chan; Daniel P Depledge; Vladyslav Nikolayevskyy; Agnieszka Broda; Madeline J Stone; Mette T Christiansen; Rachel Williams; Michael B McAndrew; Helena Tutill; Julianne Brown; Mark Melzer; Caryn Rosmarin; Timothy D McHugh; Robert J Shorten; Francis Drobniewski; Graham Speight; Judith Breuer Journal: J Clin Microbiol Date: 2015-05-13 Impact factor: 5.948
Authors: Soumitesh Chakravorty; Jong Seok Lee; Eun Jin Cho; Sandy S Roh; Laura E Smith; Jiim Lee; Cheon Tae Kim; Laura E Via; Sang-Nae Cho; Clifton E Barry; David Alland Journal: J Clin Microbiol Date: 2014-10-22 Impact factor: 5.948
Authors: Diana Machado; Tatiane S Coelho; João Perdigão; Catarina Pereira; Isabel Couto; Isabel Portugal; Raquel De Abreu Maschmann; Daniela F Ramos; Andrea von Groll; Maria L R Rossetti; Pedro A Silva; Miguel Viveiros Journal: Front Microbiol Date: 2017-04-27 Impact factor: 5.640