Literature DB >> 235294

Lysine-ketoglutarate reductase in human tissues.

J Hutzler, J Dancis.   

Abstract

Lysine-ketoglutarate reductase (saccharopine dehydrogenase (NADP+, lysine-forming) EC 1.5.1.8) from human liver has been partially purified and characterized. A spectrophotometric assay is described. The Michaelis constants have been determined for lysine (1.5-10-3 M), alpha-ketoglutarate (1-10-3 M) and NADPH (8-10-5 M). The pH optimum is 7.8. The enzyme is product inhibited. The specificity of the enzyme, response to inhibitors, pH and thermal stability are reported. Lysine-ketoglutarate reductase is present in high concentration in liver and heart, to a lesser degree in kidney and skin and in trace amounts in several other tissues. Saccharopine dehydrogenase (saccharopine dehydrogenase (NAD+, L-glutamate-forming) EC 1.5.1.9) was demonstrable only in liver and kidney. Lysine-ketoglutarate reductase reacts effectively with delta-hydroxylysine.

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Year:  1975        PMID： 235294     DOI： 10.1016/0005-2744(75)90284-3

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  17 in total

1.  The conversion of lysine into piperidine, cadaverine, and pipecolic acid in the brain and other organs of the mouse.

Authors:  T Schmidt-Glenewinkel; Y Nomura; E Giacobini
Journal:  Neurochem Res       Date:  1977-12       Impact factor: 3.996

2.  A new type of hyperlysinaemia due to a transport defect of lysine into mitochondria.

Authors:  K Oyanagi; T Aoyama; A Tsuchiyama; T Nakao; N Uetsuji; K Wagatsuma; S Tsugawa
Journal:  J Inherit Metab Dis       Date:  1986       Impact factor: 4.982

3.  Comparison of lysine and tryptophan catabolizing enzymes in rat and bovine tissues.

Authors:  A Mukhopadhyay; S M Mungre; D R Deshmukh
Journal:  Experientia       Date:  1990-08-15

4.  An economically and environmentally acceptable synthesis of chiral drug intermediate L-pipecolic acid from biomass-derived lysine via artificially engineered microbes.

Authors:  Jie Cheng; Yuding Huang; Le Mi; Wujiu Chen; Dan Wang; Qinhong Wang
Journal:  J Ind Microbiol Biotechnol       Date:  2018-05-10       Impact factor: 3.346

5.  Lysine degradation through the saccharopine pathway in mammals: involvement of both bifunctional and monofunctional lysine-degrading enzymes in mouse.

Authors:  F Papes; E L Kemper; G Cord-Neto; F Langone; P Arruda
Journal:  Biochem J       Date:  1999-12-01       Impact factor: 3.857

6.  Lysine kinetics in preterm infants: the importance of enteral feeding.

Authors:  S R D van der Schoor; P J Reeds; F Stellaard; J D L Wattimena; P J J Sauer; H A Büller; J B van Goudoever
Journal:  Gut       Date:  2004-01       Impact factor: 23.059

Review 7.  Lysine metabolism in mammalian brain: an update on the importance of recent discoveries.

Authors:  André Hallen; Joanne F Jamie; Arthur J L Cooper
Journal:  Amino Acids       Date:  2013-09-17       Impact factor: 3.520

8.  Blood-brain barrier transport of L-pipecolic acid in various rat brain regions.

Authors:  A K Charles; Y F Chang; N R Myslinski
Journal:  Neurochem Res       Date:  1983-09       Impact factor: 3.996

9.  Lysine metabolism in the human and the monkey: demonstration of pipecolic acid formation in the brain and other organs.

Authors:  Y F Chang
Journal:  Neurochem Res       Date:  1982-05       Impact factor: 3.996

10.  The mitochondrial pool of free amino acids reflects the composition of mitochondrial DNA-encoded proteins: indication of a post- translational quality control for protein synthesis.

Authors:  Catherine Ross-Inta; Chern-Yi Tsai; Cecilia Giulivi
Journal:  Biosci Rep       Date:  2008-10       Impact factor: 3.840
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