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Literature DB >> 23526173

Quantifying CFSE Label Decay in Flow Cytometry Data.

H T Banks¹, A Choi, T Huffman, J Nardini, L Poag, W C Thompson.

Abstract

We developed a series of models for the label decay in cell proliferation assays when the intracellular dye carboxyfluorescein succinimidyl ester (CFSE) is used as a staining agent. Data collected from two healthy patients were used to validate the models and to compare the models with the Akiake Information Criteria. The distinguishing features of multiple decay rates in the data are readily characterized and explained via time dependent decay models such as the logistic and Gompertz models.

Entities: Chemical Disease Gene Species

Keywords: Akiake Information; Gompertz growth; Michaelis-Menten kinetics; carboxyfluorescein succinimidyl ester (CFSE); exponential decay; inverse problems; logistic growth; ordinary differential equation models

Year: 2013 PMID： 23526173 PMCID： PMC3601764 DOI： 10.1016/j.aml.2012.12.010

Source DB: PubMed Journal: Appl Math Lett ISSN： 0893-9659 Impact factor: 4.055

16 in total

Review 1. Fluorescent dyes for lymphocyte migration and proliferation studies.

Authors: C R Parish
Journal: Immunol Cell Biol Date: 1999-12 Impact factor: 5.126

2. A cellular calculus for signal integration by T cells.

Authors: A V Gett; P D Hodgkin
Journal: Nat Immunol Date: 2000-09 Impact factor: 25.606

3. New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes.

Authors: Benjamin J C Quah; Christopher R Parish
Journal: J Immunol Methods Date: 2012-02-21 Impact factor: 2.303

4. Heterogeneous cell response to topotecan in a CFSE-based proliferation test.

Authors: Giada Matera; Monica Lupi; Paolo Ubezio
Journal: Cytometry A Date: 2004-12 Impact factor: 4.355

5. Distributed parameter identification for a label-structured cell population dynamics model using CFSE histogram time-series data.

Authors: Tatyana Luzyanina; Dirk Roose; Gennady Bocharov
Journal: J Math Biol Date: 2008-12-19 Impact factor: 2.259

Quantifying CFSE Label Decay in Flow Cytometry Data.

Review 1. Fluorescent dyes for lymphocyte migration and proliferation studies.

2. A cellular calculus for signal integration by T cells.

3. New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes.

4. Heterogeneous cell response to topotecan in a CFSE-based proliferation test.

5. Distributed parameter identification for a label-structured cell population dynamics model using CFSE histogram time-series data.

6. A new model for the estimation of cell proliferation dynamics using CFSE data.

7. A division-dependent compartmental model for computing cell numbers in CFSE-based lymphocyte proliferation assays.

8. Determination of lymphocyte division by flow cytometry.

9. Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling.

10. Numerical modelling of label-structured cell population growth using CFSE distribution data.

1. Mathematical models for CFSE labelled lymphocyte dynamics: asymmetry and time-lag in division.

2. Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS.

3. A novel statistical analysis and interpretation of flow cytometry data.

4. Storage-Induced Micro-Erythrocytes Can Be Quantified and Sorted by Flow Cytometry.

Review 5. Asymmetry of Cell Division in CFSE-Based Lymphocyte Proliferation Analysis.