| Literature DB >> 2351837 |
J P Buyon1, S G Slade, E K Chan, E M Tan, R Winchester.
Abstract
A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide 30:0.8, ratio = 37.5. A higher ratio of monomer to cross-linker, ratio = 172.4 used in a 15% acrylamide solution, readily separates the two peptide chains. This method facilitates the detection of the 52 kDa SSA/Ro component which otherwise might have been incorrectly assigned as a 48 kDa SSB/La polypeptide.Entities:
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Year: 1990 PMID： 2351837 DOI： 10.1016/0022-1759(90)90440-7
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303