OBJECTIVE: Fungal infections are potential public health threats all over the world. In the present study, effect of Matricaria recutita flower essential oil (EO) was evaluated against medically important dermatophytes and opportunistic saprophytes using microbioassay technique. MATERIALS AND METHODS: Flower essential oil (EO) of M. recutita prepared by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS). The effect of plant EO on the growth of pathogenic dermatophytes and opportunistic saprophytes was assessed using microbioassay technique. In the bioassay, fungi were cultured in 6-well flat-bottom microplates in presence of various concentrations of plant EO (2.5-1000μg/mL) for 4-10days at 28°C. RESULTS: A total of 14 compounds were identified in the plant oil by GC/MS accounting for 97.5% of the oil composition. The main compound identified was chamazulene (61.3%) followed by isopropyl hexadecanoate (12.7%), trans-trans-farnesol (6.9%) and E-β-farnesol (5.2%). Growth inhibition for the dermatophytes exposed to serial two-fold concentrations of plant EO (2.5 to 80μg/mL) was reported in the range of 3.24 to 68.15% for Microsporum gypseum, 24.48 to 100% for M. canis, 11.40 to 96.65% for Trichophyton mentagrophytes, 27.79 to 100% for T. rubrum and 45.73 to 100% for T. tonsurans. M. recutita EO inhibited the growth of opportunistic saprophytes by 3.98 to 64.29% for Aspergillus flavus, 6.38 to 93.62% for A. fumigatus, 3.52 to 89.45% for A. niger, 6.38 to 77.66% for Trichoderma harzianum and 17.41 to 89.41% for Fusarium oxysporum in serial two-fold concentrations of 15.62 to 1000μg/mL. CONCLUSION: Results of the present study indicate that M. recutita could be considered as a potential candidate for designing effective antifungal formulations suitable for treatment of dermatophytosis and other fungal infections.
OBJECTIVE:Fungal infections are potential public health threats all over the world. In the present study, effect of Matricaria recutita flower essential oil (EO) was evaluated against medically important dermatophytes and opportunistic saprophytes using microbioassay technique. MATERIALS AND METHODS:Flower essential oil (EO) of M. recutita prepared by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS). The effect of plant EO on the growth of pathogenic dermatophytes and opportunistic saprophytes was assessed using microbioassay technique. In the bioassay, fungi were cultured in 6-well flat-bottom microplates in presence of various concentrations of plant EO (2.5-1000μg/mL) for 4-10days at 28°C. RESULTS: A total of 14 compounds were identified in the plant oil by GC/MS accounting for 97.5% of the oil composition. The main compound identified was chamazulene (61.3%) followed by isopropyl hexadecanoate (12.7%), trans-trans-farnesol (6.9%) and E-β-farnesol (5.2%). Growth inhibition for the dermatophytes exposed to serial two-fold concentrations of plant EO (2.5 to 80μg/mL) was reported in the range of 3.24 to 68.15% for Microsporum gypseum, 24.48 to 100% for M. canis, 11.40 to 96.65% for Trichophyton mentagrophytes, 27.79 to 100% for T. rubrum and 45.73 to 100% for T. tonsurans. M. recutita EO inhibited the growth of opportunistic saprophytes by 3.98 to 64.29% for Aspergillus flavus, 6.38 to 93.62% for A. fumigatus, 3.52 to 89.45% for A. niger, 6.38 to 77.66% for Trichoderma harzianum and 17.41 to 89.41% for Fusarium oxysporum in serial two-fold concentrations of 15.62 to 1000μg/mL. CONCLUSION: Results of the present study indicate that M. recutita could be considered as a potential candidate for designing effective antifungal formulations suitable for treatment of dermatophytosis and other fungal infections.
Authors: Soumaya Hajaji; Ines Sifaoui; Atteneri López-Arencibia; María Reyes-Batlle; Ignacio A Jiménez; Isabel L Bazzocchi; Basilio Valladares; Hafidh Akkari; Jacob Lorenzo-Morales; José E Piñero Journal: Parasitol Res Date: 2018-06-28 Impact factor: 2.289
Authors: Hosein Sharifi; Mohammad Bagher Minaie; Mohammad Javad Qasemzadeh; Nematollah Ataei; Mohammad Gharehbeglou; Mojtaba Heydari Journal: J Evid Based Complementary Altern Med Date: 2015-09-30
Authors: Rafael Alberto Martínez-Díaz; Alexandra Ibáñez-Escribano; Jesús Burillo; Lorena de Las Heras; Gema Del Prado; M Teresa Agulló-Ortuño; Luis F Julio; Azucena González-Coloma Journal: Mem Inst Oswaldo Cruz Date: 2015-06-24 Impact factor: 2.743