Histoplasmosis mimicking primary lung cancer or pulmonary metastases.

OBJECTIVE: To describe the main clinical and radiological characteristics of patients with histoplasmosis mimicking lung cancer.
METHODS: This was a retrospective descriptive study based on the analysis of the medical records of the 294 patients diagnosed with histoplasmosis between 1977 and 2011 at the Mycology Laboratory of the Santa Casa Sisters of Mercy Hospital of Porto Alegre in the city of Porto Alegre, Brazil. The diagnosis of histoplasmosis was established by culture, histopathological examination, or immunodiffusion testing (identification of M or H precipitation bands). After identifying the patients with macroscopic lesions, as well as radiological and CT findings consistent with malignancy, we divided the patients into two groups: those with a history of cancer and presenting with lesions mimicking metastases (HC group); and those with no such history but also presenting with lesions mimicking metastases (NHC group).
RESULTS: Of the 294 patients diagnosed with histoplasmosis, 15 had presented with lesions mimicking primary neoplasia or metastases (9 and 6 in the HC and NHC groups, respectively). The age of the patients ranged from 13 to 67 years (median, 44 years). Of the 15 patients, 14 (93%) presented with pulmonary lesions at the time of hospitalization.
CONCLUSIONS: The clinical and radiological syndrome of neoplastic disease is not confined to malignancy, and granulomatous infectious diseases must therefore be considered in the differential diagnosis.

Introduction

Infection with the thermally dimorphic fungus Histoplasma capsulatum var. capsulatum (H. capsulatum) can produce a broad spectrum of clinical manifestations, ranging from self-limiting respiratory complaints to progressive, life-threatening infections. Primary pulmonary infection results from the inhalation of airborne microconidia, and the vast majority of infections are self-limiting-in most cases, the infection is limited to the lungs-however, since the onset of the AIDS epidemic, disseminated histoplasmosis has been reported with greater frequency.( , )

Air currents can carry the microconidia for miles, exposing unsuspecting individuals to indirect contact with a contaminated site. In addition, the fungus can be present at environmental sites that are not visibly contaminated with droppings from birds or bats, thus reducing the chance that histoplasmosis will be suspected.( ) In Brazil, histoplasmosis is a recurrent topic in pulmonology.( - ) A review of the literature revealed that there have been cases of histoplasmosis mimicking malignancy.( - ) The rarity of this manifestation prompted our study, the objective of which was to describe the main clinical and radiological characteristics of patients with histoplasmosis mimicking cancer. We also review the literature on the topic.

Methods

This was a retrospective descriptive study based on the analysis of the medical records of the 294 patients diagnosed with histoplasmosis between 1977 and 2011 at the Mycology Laboratory of the Santa Casa Sisters of Mercy Hospital of Porto Alegre in the city of Porto Alegre, Brazil. The study was approved by the Human Research Ethics Committee of the Hospital (Protocol no. 306/11). The diagnosis of histoplasmosis was established by culture, histopathological examination, or immunodiffusion testing (identification of M or H precipitation bands).( )

After identifying the patients with macroscopic lesions, as well as with radiological and CT findings consistent with malignancy, we divided the patients into two groups: patients with a history of cancer presenting lesions mimicking metastases (HC group); and patients without a history of cancer presenting at least one lesion mimicking primary cancer (NHC group). The radiological criteria for inclusion in the groups were the following: having multiple lesions; having a higher prevalence of pulmonary lesions in the lower lobes (HC group); or having a solid solitary lesion with homogenous soft-tissue attenuation and without benign calcification or spiculated margins.

Results

Of the 294 patients diagnosed with histoplasmosis, 15 presented with lesions mimicking primary neoplasia or simulating metastases. The most common clinical symptoms were fever, cough, weight loss, and chest pain. Five patients were asymptomatic. Of the 15 patients, 9 had a history of cancer and presented with lesions simulating metastases (HC group; Figure 1), and 6 patients had no history of cancer and presented with at least one lesion mimicking primary malignancy (NHC group; Figure 2).

Figure 1

Pulmonary histoplasmosis in a 29-year-old female patient with a history of melanoma and treatment with antineoplastic agents (patient 5). The CT scans reveal multiple, sharply circumscribed, randomly distributed nodules, predominantly in the left lung.

Figure 2

Histoplasmoma in a 66-year-old male patient with arterial hypertension (patient 11). In A, a posteroanterior chest X-ray and, in B, a CT scan of the chest revealing a homogeneous consolidation (3 cm in diameter) in the upper segment of the right lower lobe.

Clinical, demographic, and biochemical findings are shown in Table 1. The age of the patients ranged from 13 to 67 years (median, 44 years). Of the 15 patients, 14 (93%) presented with pulmonary lesions at the time of hospitalization. In all 15 patients, the diagnosis of histoplasmosis was made by demonstrating oval budding yeasts, typical of H. capsulatum, on biopsy specimens stained with methenamine silver. The diagnosis was confirmed by culture in only 4 cases, although culture was requested in only 5. Seven patients were tested for the presence of specific antibodies to H. capsulatum, and 6 of those 7 tested negative.

Table 1

Clinical findings, radiological findings, diagnoses, treatment, and outcomes of the 15 patients with histoplasmosis mimicking primary or metastatic lung cancer.

Patients with a history of cancer
Patient	Age, years/Gender	Primary disease	Clinical presentation	Chest X-ray findings	Site(s) of infection	Diagnosis	Treatment	Outcome
Hist	IDh	Cult
1	58/M	Multiple myeloma	Disseminated	Consolidation	Lung, mediastinal lymph node, adrenal gland	+	ND	ND	Untreated	Died
2	40/F	Carcinoid tumor	Pulmonary/histoplasmoma	Nodules	Lung	+	−	ND	Surgery	Improved
3	32/M	Lymphoma	Disseminated	Mass	Lung, skin	+	+	+	Untreated	Died
4	67/M	Squamous cell carcinoma	Pulmonary/histoplasmoma	Mass, consolidation	Lung	+	−	ND	Itraconazole	Improved
5	29/F	Melanoma	Pulmonary	Nodules	Lung	+	−	ND	Not reported	Not reported
6	13/F	Lymphoma	Disseminated	Nodules	Lung, larynx	+	ND	ND	Itraconazole	Improved
7	50/F	Pancreatic adenocarcinoma	Disseminated	Nodules	Lung, pleura	+	ND	+	Untreated	Died
8	58/M	Lymphoma	Disseminated	Pulmonary infiltrates	Lung	+	ND	+	Not reported	Not reported
9	54/F	Breast carcinoma	Pulmonary	Mass	Lung	+	ND	ND	Surgery	Improved
Patients without a history of cancer
Patient	Age, years/Gender	Primary disease	Clinical presentation	Chest radiographic findings	Site(s) of infection	Diagnosis	Treatment	Outcome
Hist	IDh	Cult
10	25/M	AIDS	Disseminated	Normal	Pharynx, skin	+	ND	ND	Amphotericin B	Improved
11	66/M	Arterial hypertension	Pulmonary/histoplasmoma	Mass	Lung, lymph node	+	−	ND	Itraconazole	Improved
12	63/F	None	Pulmonary/histoplasmoma	Mass	Lung	+	−	ND	Surgery	Improved
13	25/F	None	Pulmonary/histoplasmoma	Mass	Lung	+	−	+	Itraconazole	Improved
14	13/M	None	Disseminated	Nodule	Lung, mediastinal	+	ND	ND	Itraconazole	Improved
15	67/M	Liver transplant	Pulmonary	Mass	Lung	+	ND	−	Not reported	Not reported

: histopathology, tissue section (H&E and Grocott-Gomori methenamine silver);

: immunodiffusion for Histoplasma capsulatum;

: culture on Sabouraud dextrose agar and Mycosel(r);

: not determined.

Discussion

The results of the present study underscore the fact that the clinical syndrome of neoplastic disease is not confined to malignancy and that granulomatous infectious diseases must be considered in the differential diagnosis. Excluding histoplasmosis from the differential diagnosis of pulmonary lesions can delay the diagnostic process.

There are sporadic reports of patients with histoplasmosis that have been misdiagnosed as having head and neck cancer,( , ) primary lung cancer,( - ) or lymphoma.( , ) In such cases, the cytological examination of the pleural fluid can suggest neoplasia, because giant cells in which H. capsulatum is present could be mistaken for malignant lymphoblasts.( ) It is of interest that histoplasmosis granuloma might be associated with a type of lung carcinoma.( ) On CT scans, as well as on scans made with more advanced imaging techniques, such as positron emission tomography, histoplasmosis can mimic malignant lesions.

Immunocompromised patients, such as those in our HC group, are more susceptible to disseminated disease. In the literature, approximately two-thirds of patients with chronic disseminated histoplasmosis present with oropharyngeal or laryngeal involvement, which is almost invariably the clinical feature that leads to the diagnosis,( ) and one of our patients (patient 6) presented with this type of lesion. In addition, adrenal involvement has been found in over half of all patients with disseminated histoplasmosis, the lesions being more commonly found in the zona reticularis; this could be due to the presence of higher downstream concentrations of cortisol, en route from secretion to the medullary central venous system.( ) Three of the patients in the HC group had disseminated disease. Of those 3, only 1 was submitted to autopsy (case 1), which showed adrenal involvement. Cutaneous lesions of disseminated histoplasmosis are infrequent, being most commonly found in individuals who are infected with HIV,( ) which nearly none of our patients were.

The patients in the NHC group demonstrated that histoplasmosis could occur in individuals who are not apparently immunocompromised. Because imaging findings often mimic other granulomatous infections and neoplastic processes, they are not considered diagnostic.( ) Histoplasmosis in the mediastinum is an uncommon diagnosis that has a presentation similar to that of other benign and neoplastic conditions encountered in the chest.( ) The principle that a solitary circumscribed pulmonary nodule is diagnostic of carcinoma unless proven otherwise has been well established (patients 4, 11, 12, and 13), especially in cases of enlarging histoplasmoma.( )

The diagnostic approach depends on the type of infection and the amount of microconidia inhaled. A variety of tests, including culture, specific staining for fungal cells, antigen detection, and serologic tests for antibodies, are used for the diagnosis of histoplasmosis. The sensitivity of laboratory tests depends on the clinical manifestation of histoplasmosis (disseminated, chronic pulmonary, or self-limiting).( ) We observed these differences and the importance of using more than one diagnostic method. In our cohort, the patients with histoplasmoma (patients 11 and 12) showed no specific antibodies to H. capsulatum; this also can happen in immunocompromised patients with positive cultures.( ) This information can be helpful because the absence of specific antibodies is usually associated with the absence of infection in immunocompetent patients.

In conclusion, histoplasmosis should be considered in the differential diagnosis of primary and metastatic lung cancer, because delaying treatment can allow severe manifestations of the former to occur.