Literature DB >> 2350348

Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta).

N Matsukawa1, Y Nonaka, Z Ying, J Higaki, T Ogihara, M Okamoto.   

Abstract

Two distinct forms of cDNA encoding rat aldosterone synthase were cloned from an adrenal capsular tissue cDNA library. The deduced amino acid sequences showed that one of the enzymes (P-450(11 beta),aldo-1) had a long extension peptide composed of 34 amino acid residues while the other (P-450(11 beta),aldo-2) had an extension peptide identical to that of rat P-450(11 beta). Glu at the 320th position of P-450(11 beta),aldo-1 was replaced with Lys in P-450(11 beta),aldo-2. The amino acid sequence of the aldosterone synthase was highly homologous (81%) to rat P-450(11 beta). Constructed expression vector containing the cDNA for extension peptide of P-450(11 beta) and the mature protein of P-450(11 beta),aldo-1 was transfected into COS-7 cells. The cells converted 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone.

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Year:  1990        PMID： 2350348     DOI： 10.1016/0006-291x(90)91460-a

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Localization of the gene transcripts of 11 beta-hydroxylase and aldosterone synthase in the rat adrenal cortex by in situ hybridization.

Authors:  M Yabu; T Senda; Y Nonaka; N Matsukawa; M Okamoto; H Fujita
Journal:  Histochemistry       Date:  1991

2.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

3.  Mutations in the human CYP11B2 (aldosterone synthase) gene causing corticosterone methyloxidase II deficiency.

Authors:  L Pascoe; K M Curnow; L Slutsker; A Rösler; P C White
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

4.  Zone-specific regulation of two messenger RNAs for P450c11 in the adrenals of pregnant and nonpregnant rats.

Authors:  M P Malee; S H Mellon
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

5.  Sodium depletion increases sympathetic neurite outgrowth and expression of a novel TMEM35 gene-derived protein (TUF1) in the rat adrenal zona glomerulosa.

Authors:  Phu V Tran; Michael K Georgieff; William C Engeland
Journal:  Endocrinology       Date:  2010-08-04       Impact factor: 4.736

6.  Blood pressure and survival of a chromosome 7 congenic strain bred from Dahl rats.

Authors:  G T Cicila; O I Dukhanina; T W Kurtz; R Walder; M R Garrett; H Dene; J P Rapp
Journal:  Mamm Genome       Date:  1997-12       Impact factor: 2.957

7.  A mutation in CYP11B1 (Arg-448----His) associated with steroid 11 beta-hydroxylase deficiency in Jews of Moroccan origin.

Authors:  P C White; J Dupont; M I New; E Leiberman; Z Hochberg; A Rösler
Journal:  J Clin Invest       Date:  1991-05       Impact factor: 14.808

8.  Glucocorticoid-suppressible hyperaldosteronism and adrenal tumors occurring in a single French pedigree.

Authors:  L Pascoe; X Jeunemaitre; M C Lebrethon; K M Curnow; C E Gomez-Sanchez; J M Gasc; J M Saez; P Corvol
Journal:  J Clin Invest       Date:  1995-11       Impact factor: 14.808

9.  Role of adrenal renin in the regulation of adrenal steroidogenesis by corticotropin.

Authors:  M Sander; D Ganten; S H Mellon
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-04       Impact factor: 11.205
  9 in total

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