| Literature DB >> 23477541 |
Hashem Akhavan-Tafti1, Dean G Binger, John J Blackwood, Ying Chen, Richard S Creager, Renuka de Silva, Robert A Eickholt, Jose E Gaibor, Richard S Handley, Kenneth P Kapsner, Senja K Lopac, Michael E Mazelis, Terri L McLernon, James D Mendoza, Bruce H Odegaard, Sarada G Reddy, Michael Salvati, Barry A Schoenfelner, Nir Shapir, Katherine R Shelly, Jeff C Todtleben, Guoping Wang, Wenhua Xie.
Abstract
A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte.Mesh：
Substances：
Year: 2013 PMID： 23477541 DOI： 10.1021/ja312039k
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419