AIM: The aim of this study was to investigate in vitro the utility of biologically compatible, nontoxic and cell-specific targetable hydrogel nanoparticles (NPs), which have Coomassie® Brilliant Blue G dye (Sigma-Aldrich, MO, USA) covalently linked into their polyacrylamide matrix, as candidates for photothermal therapy (PTT) of cancer cells. MATERIALS & METHODS: Hydrogel NPs with Coomassie Brilliant Blue G dye covalently linked into their polyacrylamide matrix were fabricated using a reverse micelle microemulsion polymerization method and were found to be 80-95 nm in diameter, with an absorbance value of 0.52. PTT-induced hyperthermia/thermolysis was achieved at 37°C using an inexpensive, portable, light-emitting diode array light source (590 nm, 25 mW/cm(2)). RESULTS & CONCLUSION: Hydrogel NPs with Coomassie Brilliant Blue G dye linked into their polyacrylamide matrix are effective in causing PTT-induced thermolysis in immortalized human cervical cancer cell line (HeLa) cells for varying NP concentrations and treatment times. These multifunctional particles have previously been used in cancer studies to enable delineation, for glioma surgery and in photoacoustic imaging studies. The addition of the PTT function would enable a three-pronged theranostic approach to cancer medicine, such as guided tumor surgery with intra-operative photoacoustic imaging and intra-operative PTT.
AIM: The aim of this study was to investigate in vitro the utility of biologically compatible, nontoxic and cell-specific targetable hydrogel nanoparticles (NPs), which have Coomassie® Brilliant Blue G dye (Sigma-Aldrich, MO, USA) covalently linked into their polyacrylamide matrix, as candidates for photothermal therapy (PTT) of cancer cells. MATERIALS & METHODS: Hydrogel NPs with Coomassie Brilliant Blue G dye covalently linked into their polyacrylamide matrix were fabricated using a reverse micelle microemulsion polymerization method and were found to be 80-95 nm in diameter, with an absorbance value of 0.52. PTT-induced hyperthermia/thermolysis was achieved at 37°C using an inexpensive, portable, light-emitting diode array light source (590 nm, 25 mW/cm(2)). RESULTS & CONCLUSION: Hydrogel NPs with Coomassie Brilliant Blue G dye linked into their polyacrylamide matrix are effective in causing PTT-induced thermolysis in immortalized human cervical cancer cell line (HeLa) cells for varying NP concentrations and treatment times. These multifunctional particles have previously been used in cancer studies to enable delineation, for glioma surgery and in photoacoustic imaging studies. The addition of the PTT function would enable a three-pronged theranostic approach to cancer medicine, such as guided tumor surgery with intra-operative photoacoustic imaging and intra-operative PTT.
Authors: Guochao Nie; Hoe Jin Hah; Gwangseong Kim; Yong-Eun Koo Lee; Ming Qin; Tanvi S Ratani; Panagiotis Fotiadis; Amber Miller; Akiko Kochi; Di Gao; Thomas Chen; Daniel A Orringer; Oren Sagher; Martin A Philbert; Raoul Kopelman Journal: Small Date: 2012-01-09 Impact factor: 13.281
Authors: Yong-Eun Lee Koo; G Ramachandra Reddy; Mahaveer Bhojani; Randy Schneider; Martin A Philbert; Alnawaz Rehemtulla; Brian D Ross; Raoul Kopelman Journal: Adv Drug Deliv Rev Date: 2006-09-28 Impact factor: 15.470
Authors: Daniel A Orringer; Thomas Chen; Dah-Luen Huang; William M Armstead; Benjamin A Hoff; Yong-Eun L Koo; Richard F Keep; Martin A Philbert; Raoul Kopelman; Oren Sagher Journal: Neurosurgery Date: 2010-04 Impact factor: 4.654
Authors: Jeffrey Hrkach; Daniel Von Hoff; Mir Mukkaram Ali; Elizaveta Andrianova; Jason Auer; Tarikh Campbell; David De Witt; Michael Figa; Maria Figueiredo; Allen Horhota; Susan Low; Kevin McDonnell; Erick Peeke; Beadle Retnarajan; Abhimanyu Sabnis; Edward Schnipper; Jeffrey J Song; Young Ho Song; Jason Summa; Douglas Tompsett; Greg Troiano; Tina Van Geen Hoven; Jim Wright; Patricia LoRusso; Philip W Kantoff; Neil H Bander; Christopher Sweeney; Omid C Farokhzad; Robert Langer; Stephen Zale Journal: Sci Transl Med Date: 2012-04-04 Impact factor: 17.956
Authors: Daniel A Orringer; Yong-Eun L Koo; Thomas Chen; Gwangseong Kim; Hoe Jin Hah; Hao Xu; Shouyan Wang; Richard Keep; Martin A Philbert; Raoul Kopelman; Oren Sagher Journal: Neurosurgery Date: 2009-05 Impact factor: 4.654