An integrated label-free cell-based biosensor for simultaneously monitoring of cellular physiology multiparameter in vitro.

The study presented a novel integrated cell-based biosensor with light-addressable potentiometric sensor (LAPS) and electrical cell-substrate impedance sensor (ECIS). The integrated cell-based biosensor was fabricated in order to monitor the cellular metabolism and growth status by LAPS and ECIS. Moreover, the specific instrument was established for controlling the detection processes. Sensor test and cell experiments were carried out to determine the performance of integrated sensor. The result showed that integrated biosensor can monitor the change of cell electrical impedance and extracellular acidification simultaneously which can be used for drug evaluation by monitoring cell growth status (e.g. cell number, adhesion, and morphology) and cell energy metabolism status (e.g. extracellular acidification) in real time. With the development of sensor technology, the integrated cell-based biosensor will be a utility platform to study the mechanism of cellular metabolism and in vitro drug analysis.