Nozomi Takai1, Yukari Tanaka, Ayahisa Watanabe, Hideo Saji. 1. Drug Metabolism & Pharmacokinetics, Drug Developmental Research Laboratories, Shionogi & Co. Ltd., 3-1-1 Futaba, Toyonaka, Osaka 561-0825, Japan. nozomi.takai@shionogi.co.jp
Abstract
BACKGROUND: Therapeutic peptides and proteins are being increasingly explored as potential therapeutic agents for molecular-targeted therapy, and the requirement for quantitative bioanalytical tools for such molecules has been discussed. RESULTS: The distribution of octreotide, a synthetic octapeptide analog of somatostatin, in the liver and kidney of mice administered with the analog was clearly visualized by MALDI-Imaging MS (IMS). The developed MALDI-IMS analytical method successfully quantified the amount of octreotide on tissue sections (accuracy was 76-127%) and the 2,5-dihydroxybenzoic acid-based normalization method was effective. CONCLUSION: The results of this study suggest that MALDI-IMS enables the quantification of an administered therapeutic peptide on biological tissue sections, as well as visualization of the in vivo distribution of the peptide.
BACKGROUND: Therapeutic peptides and proteins are being increasingly explored as potential therapeutic agents for molecular-targeted therapy, and the requirement for quantitative bioanalytical tools for such molecules has been discussed. RESULTS: The distribution of octreotide, a synthetic octapeptide analog of somatostatin, in the liver and kidney of mice administered with the analog was clearly visualized by MALDI-Imaging MS (IMS). The developed MALDI-IMS analytical method successfully quantified the amount of octreotide on tissue sections (accuracy was 76-127%) and the 2,5-dihydroxybenzoic acid-based normalization method was effective. CONCLUSION: The results of this study suggest that MALDI-IMS enables the quantification of an administered therapeutic peptide on biological tissue sections, as well as visualization of the in vivo distribution of the peptide.
Authors: Ignacy Rzagalinski; Nadine Hainz; Carola Meier; Thomas Tschernig; Dietrich A Volmer Journal: J Am Soc Mass Spectrom Date: 2018-02-07 Impact factor: 3.109