Literature DB >> 23423900

Rational protein sequence diversification by multi-codon scanning mutagenesis.

Jia Liu1, T Ashton Cropp.   

Abstract

A new method for protein sequence diversification is based on generating random codon mutations to an encoding DNA. This allows for the scanning of user-defined amino acid changes to any protein of interest, and is an alternative to traditional directed evolution strategies. This chapter describes the procedures required to apply this technology to any protein of interest. The resulting libraries can then be screened for new or improved protein function.

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Year:  2013        PMID： 23423900      PMCID： PMC4104598          DOI： 10.1007/978-1-62703-293-3_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications.

Authors:  S Haapa; S Taira; E Heikkinen; H Savilahti
Journal:  Nucleic Acids Res       Date:  1999-07-01       Impact factor: 16.971

2.  User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries.

Authors:  Wayne M Patrick; Andrew E Firth; Jonathan M Blackburn
Journal:  Protein Eng       Date:  2003-06

3.  A second-generation system for unbiased reading frame selection.

Authors:  Monica L Gerth; Wayne M Patrick; Stefan Lutz
Journal:  Protein Eng Des Sel       Date:  2004-08-25       Impact factor: 1.650

4.  A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons.

Authors:  Jia Liu; T Ashton Cropp
Journal:  Protein Eng Des Sel       Date:  2011-12-18       Impact factor: 1.650

5.  Heat inactivation of DNA ligase prior to electroporation increases transformation efficiency.

Authors:  S Ymer
Journal:  Nucleic Acids Res       Date:  1991-12-25       Impact factor: 16.971

6.  N-terminal domain-deleted mu transposase exhibits increased transposition activity with low target site preference in modified buffers.

Authors:  Yun Cheol Kim; Sherie L Morrison
Journal:  J Mol Microbiol Biotechnol       Date:  2008-11-25

7.  Transformation of Escherichia coli increases 260-fold upon inactivation of T4 DNA ligase.

Authors:  B K Michelsen
Journal:  Anal Biochem       Date:  1995-02-10       Impact factor: 3.365

8.  Probing the alpha-complementing domain of E. coli beta-galactosidase with use of an insertional pentapeptide mutagenesis strategy based on Mu in vitro DNA transposition.

Authors:  Eini Poussu; Mauno Vihinen; Lars Paulin; Harri Savilahti
Journal:  Proteins       Date:  2004-03-01

9.  Amplification of complex gene libraries by emulsion PCR.

Authors:  Richard Williams; Sergio G Peisajovich; Oliver J Miller; Shlomo Magdassi; Dan S Tawfik; Andrew D Griffiths
Journal:  Nat Methods       Date:  2006-07       Impact factor: 28.547
  9 in total
  1 in total

1.  Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations.

Authors:  Shu-su Liu; Xuan Wei; Xue Dong; Liang Xu; Jia Liu; Biao Jiang
Journal:  BMC Biochem       Date:  2015-07-25       Impact factor: 4.059
  1 in total

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