Literature DB >> 23422075

The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity.

Ken-ichi Yoritsune1, Tomohiko Matsuzawa, Takao Ohashi, Kaoru Takegawa.   

Abstract

N-Glycan from the fission yeast Schizosaccharomyces pombe contains outer-chain pyruvic acid 4,6-ketal-linked galactose (PvGal). Here, we characterized a putative S. pombe pyruvyltransferase, Pvg1p, reported to be essential for biosynthesis of PvGal. When p-nitrophenyl-β-Gal (pNP-β-Gal) was used as a substrate, the structure of the recombinant Pvg1p product was determined to be pNP-PvGal by one- and two-dimensional NMR spectroscopy. The recombinant Pvg1p transferred pyruvyl residues from phosphoenolpyruvate specifically to β-linked galactose.
Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Year:  2013        PMID： 23422075     DOI： 10.1016/j.febslet.2013.02.016

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

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6.  Anionic and zwitterionic moieties as widespread glycan modifications in non-vertebrates.

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Journal:  Glycoconj J       Date:  2019-07-05       Impact factor: 2.916

7.  Assaying Paenibacillus alvei CsaB-Catalysed Ketalpyruvyltransfer to Saccharides by Measurement of Phosphate Release.

Authors:  Fiona F Hager-Mair; Cordula Stefanović; Charlie Lim; Katharina Webhofer; Simon Krauter; Markus Blaukopf; Roland Ludwig; Paul Kosma; Christina Schäffer
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8.  A rationally engineered yeast pyruvyltransferase Pvg1p introduces sialylation-like properties in neo-human-type complex oligosaccharide.

Authors:  Yujiro Higuchi; Sho Yoshinaga; Ken-Ichi Yoritsune; Hiroaki Tateno; Jun Hirabayashi; Shin-Ichi Nakakita; Miho Kanekiyo; Yoshimitsu Kakuta; Kaoru Takegawa
Journal:  Sci Rep       Date:  2016-05-19       Impact factor: 4.379

9.  Functional Characterization of Enzymatic Steps Involved in Pyruvylation of Bacterial Secondary Cell Wall Polymer Fragments.

Authors:  Fiona F Hager; Arturo López-Guzmán; Simon Krauter; Markus Blaukopf; Mathias Polter; Inka Brockhausen; Paul Kosma; Christina Schäffer
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  9 in total

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