| Literature DB >> 23416182 |
Jenny Lee1, Shalini Gupta, Jin-Sheng Huang, Lasanthi P Jayathilaka, Bao-Shiang Lee.
Abstract
A strategy using reversed-phase high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), mass spectrometry (MS), nuclear magnetic resonance (NMR), chemical synthesis, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay to identify allicin as the active anticancer compound in aqueous garlic extract (AGE) is described. Changing the pH of AGE from 7.0 to 5.0 eliminated interfering molecules and enabled a clean HPLC separation of the constituents in AGE. MTT assay of the HPLC fractions identified an active fraction. Further analysis by TLC, MS, and NMR verified the active HPLC fraction as allicin. Chemically synthesized allicin was used to provide further confirmation. The results clearly identify the active compound in AGE as allicin.Entities:
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Year: 2013 PMID： 23416182 DOI： 10.1016/j.ab.2013.01.033
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365