Zebrafish are popular models for biological discovery. For investigators of the auditory and vestibular periphery, manipulations of hair cell and synaptic mechanisms have relied on inferences from extracellular recordings of physiological activity. We now provide data showing that hair cells and supporting cells of the lateral line can be directly patch-clamped, providing the first recordings of ionic channel activity, synaptic vesicle release, and gap junctional coupling in the neuromasts of living fish. Such capabilities will allow more detailed understanding of mechano-sensation of the zebrafish.
pan class="Species">Zebrafish are popn>ular models for biological discovery. For investigators of the auditory and vestibular periphery, manipulations of hair cell and synaptic mechanisms have relied on inferences from extracellular recordings of physiological activity. We now provide data showing that hair cells and supporting cells of the lateral line can be directly patch-clampn>ed, providing the first recordings of ionic channel activity, synaptic vesicle release, and gap junctional coupling in the neuromasts of living fish. Such capabilities will allow more detailed understanding of mechano-sensation of the pan class="Species">zebrafish.
Authors: Caixia Lv; William J Stewart; Otar Akanyeti; Courtney Frederick; Jie Zhu; Joseph Santos-Sacchi; Lavinia Sheets; James C Liao; David Zenisek Journal: Cell Rep Date: 2016-06-09 Impact factor: 9.423
Authors: Eileen L Troconis; Alexander J Ordoobadi; Thomas F Sommers; Razina Aziz-Bose; Ashley R Carter; Josef G Trapani Journal: J Physiol Date: 2016-06-27 Impact factor: 5.182