Zebrafish are popular models for biological discovery. For investigators of the auditory and vestibular periphery, manipulations of hair cell and synaptic mechanisms have relied on inferences from extracellular recordings of physiological activity. We now provide data showing that hair cells and supporting cells of the lateral line can be directly patch-clamped, providing the first recordings of ionic channel activity, synaptic vesicle release, and gap junctional coupling in the neuromasts of living fish. Such capabilities will allow more detailed understanding of mechano-sensation of the zebrafish.
n class="Species">Zebrafish are popular models for biological discovery. For inpan>vestigators of the auditory and vestibular periphery, manipulationpan>s of hair cell and synaptic mechanisms have relied onpan> inpan>ferenpan>ces from extracellular recordinpan>gs of physiological activity. We now provide data showinpan>g that hair cells and supportinpan>g cells of the lateral linpan>e can be directly patch-clamped, providinpan>g the first recordinpan>gs of ionpan>ic channel activity, synaptic vesicle release, and gap junctionpan>al couplinpan>g inpan> the neuromasts of livinpan>g fish. Such capabilities will allow more detailed understandinpan>g of mechano-senpan>sationpan> of the n class="Species">zebrafish.
Authors: Caixia Lv; William J Stewart; Otar Akanyeti; Courtney Frederick; Jie Zhu; Joseph Santos-Sacchi; Lavinia Sheets; James C Liao; David Zenisek Journal: Cell Rep Date: 2016-06-09 Impact factor: 9.423
Authors: Eileen L Troconis; Alexander J Ordoobadi; Thomas F Sommers; Razina Aziz-Bose; Ashley R Carter; Josef G Trapani Journal: J Physiol Date: 2016-06-27 Impact factor: 5.182