Literature DB >> 2340070

Formation of isoaspartate 99 in bovine and porcine somatotropins.

B N Violand1, M R Schlittler, P C Toren, N R Siegel.   

Abstract

Asparagine 99 in bovine (BST) and porcine somatotropins (PST) was converted to an isoaspartate residue during incubation at neutral or alkaline pH. Isoaspartate 99 BST or isoaspartate 99 PST was resolved from the normal somatotropin by reversed-phase high-performance liquid chromatography (HPLC). The altered peptide of residues 96-108 which contains isoaspartate 99 was detected by tryptic peptide mapping of the modified BST or PST. Amino acid sequencing, amino acid analysis, mass spectrometry, and co-elution with a chemically synthesized peptide containing isoaspartate 99 were used to demonstrate the existence of isoaspartate in the modified peptides. Peptide bond cleavage between Asn 99 and Ser 100 also occurred during incubation of BST and PST at neutral or alkaline pH. This chemically cleaved product was resolved on reversed-phase HPLC from both the isoaspartate 99 and normal somatotropin molecules.

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Year:  1990        PMID： 2340070     DOI： 10.1007/bf01024992

Source DB:  PubMed          Journal:  J Protein Chem        ISSN： 0277-8033


  24 in total

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Journal:  Eur J Biochem       Date:  1987-03-02

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Authors:  C Secchi; P A Biondi; A Negri; R Borroni; S Ronchi
Journal:  Int J Pept Protein Res       Date:  1986-09

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Authors:  E D Murray; S Clarke
Journal:  J Biol Chem       Date:  1984-09-10       Impact factor: 5.157

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Authors:  B A Johnson; E L Langmack; D W Aswad
Journal:  J Biol Chem       Date:  1987-09-05       Impact factor: 5.157

7.  The heterogeneity of bovine growth hormone. Extraction from the pituitary of components with different biological and immunological properties.

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Journal:  Biochem J       Date:  1984-03-01       Impact factor: 3.857

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Authors:  I M Ota; L Ding; S Clarke
Journal:  J Biol Chem       Date:  1987-06-25       Impact factor: 5.157

9.  Purification and characterization of pituitary bovine somatotropin.

Authors:  D C Wood; W J Salsgiver; T R Kasser; G W Lange; E Rowold; B N Violand; A Johnson; R M Leimgruber; G R Parr; N R Siegel
Journal:  J Biol Chem       Date:  1989-09-05       Impact factor: 5.157

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Authors:  Y C Meinwald; E R Stimson; H A Scheraga
Journal:  Int J Pept Protein Res       Date:  1986-07
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  14 in total

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8.  Determination of beta-isomerized aspartic acid as the corresponding alcohol.

Authors:  D A Carter; P N McFadden
Journal:  J Protein Chem       Date:  1994-01

9.  Identification of succinimide sites in proteins by N-terminal sequence analysis after alkaline hydroxylamine cleavage.

Authors:  M Y Kwong; R J Harris
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10.  Isolation and characterization of porcine somatotropin containing a succinimide residue in place of aspartate129.

Authors:  B N Violand; M R Schlittler; E W Kolodziej; P C Toren; M A Cabonce; N R Siegel; K L Duffin; J F Zobel; C E Smith; J S Tou
Journal:  Protein Sci       Date:  1992-12       Impact factor: 6.725
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