| Literature DB >> 2339788 |
Abstract
Ten oligomannose-type sugar chains (ManGlcNAc2-Man5GlcNAc2) were prepared from various glycoproteins and fluorescence labeled with 2-aminopyridine. The fluorescent pyridylamino (PA)-sugar chains were first separated into five fractions according to their molecular sizes by HPLC on a TSK gel Amide-80 column. Each fraction was then separated into the component PA-sugar chains by reversed-phase HPLC on a Capcell Pak C18 column according to their chemical structures. The method is useful for studying the substrate specificities of alpha-mannosidases with Man5GlcNAc2-PA as a substrate.Entities:
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Year: 1990 PMID： 2339788 DOI： 10.1016/0003-2697(90)90303-q
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365