OBJECTIVE: The present paper investigated possible correlations between the clinical presentation of hepatitis B and the TNF-α -308G/A, IFN-γ +874A/T, TGF-beta1 -509C/T, and IL-10 -1081A/G polymorphisms and associated serum levels of these cytokines. METHODS: Fifty-three hepatitis patients were selected and divided into two groups: A - inactive (n=30) and B - chronic hepatitis/cirrhosis (n=23). The control group consisted of 100 subjects who were positive for anti-HBc and anti-HBs. The serum concentrations of the cytokines were determined by immunoenzymatic assays. The polymorphisms of the cytokines genes were assessed by PCR and PCR-SSP. RESULTS: The mean serum levels of IFN-γ of the control group were significantly higher than those of groups A and B, whereas the mean levels TGF-beta1 were significantly higher in groups A and B in comparison with the control. In the case of IL-10, the mean serum level recorded in the control group was significantly higher than that of group B. The TNF-α -308AG genotype was considerably more frequent in group B (43.3%) than the control (14.4%). CONCLUSION: Higher serum levels of IFN-γ and TGF-beta1 were associated with chronic hepatitis B, and lower serum levels of IL-10 were found in patients with the active disease. Furthermore the presence of allele A of the TNF-α -308 polymorphism suggest a risk of the progressive disease.
OBJECTIVE: The present paper investigated possible correlations between the clinical presentation of hepatitis B and the TNF-α -308G/A, IFN-γ +874A/T, TGF-beta1-509C/T, and IL-10-1081A/G polymorphisms and associated serum levels of these cytokines. METHODS: Fifty-three hepatitispatients were selected and divided into two groups: A - inactive (n=30) and B - chronic hepatitis/cirrhosis (n=23). The control group consisted of 100 subjects who were positive for anti-HBc and anti-HBs. The serum concentrations of the cytokines were determined by immunoenzymatic assays. The polymorphisms of the cytokines genes were assessed by PCR and PCR-SSP. RESULTS: The mean serum levels of IFN-γ of the control group were significantly higher than those of groups A and B, whereas the mean levels TGF-beta1 were significantly higher in groups A and B in comparison with the control. In the case of IL-10, the mean serum level recorded in the control group was significantly higher than that of group B. The TNF-α -308AG genotype was considerably more frequent in group B (43.3%) than the control (14.4%). CONCLUSION: Higher serum levels of IFN-γ and TGF-beta1 were associated with chronic hepatitis B, and lower serum levels of IL-10 were found in patients with the active disease. Furthermore the presence of allele A of the TNF-α -308 polymorphism suggest a risk of the progressive disease.
Authors: Ednelza da Silva Graça Amoras; Samara Tatielle Monteiro Gomes; Felipe Bonfim Freitas; Bárbara Brasil Santana; Geraldo Ishak; Marialva Tereza Ferreira de Araújo; Sâmia Demachki; Simone Regina Souza da Silva Conde; Marluísa de Oliveira Guimarães Ishak; Ricardo Ishak; Antonio Carlos Rosário Vallinoto Journal: PLoS One Date: 2016-05-31 Impact factor: 3.240
Authors: Luiz Fernando Almeida Machado; Ricardo Roberto de Souza Fonseca; Maria Alice Freitas Queiroz; Aldemir Branco Oliveira-Filho; Izaura Maria Vieira Cayres-Vallinoto; Antonio Carlos Rosário Vallinoto; Marluísa de Oliveira Guimarães Ishak; Ricardo Ishak Journal: Viruses Date: 2021-05-07 Impact factor: 5.048