BACKGROUND: Xenon has profound neuroprotective effects after neurological injury and is currently undergoing phase 2 clinical trials in cardiac arrest patients. However, xenon is very costly, which might preclude its widespread use. We hypothesized argon, which is more available, might also protect central nervous tissues and allow better functional recovery in a rodent model of global cerebral ischaemia. METHODS: Fourteen male Sprague-Dawley rats were subjected to 7 min of cardiac arrest and 3 min of cardiopulmonary resuscitation (CPR). One hour after successful CPR, animals were randomized to either ventilation with 70% argon in oxygen (n = 7) for 1 h or 70% nitrogen (controls, n=7). A neurological deficit score (NDS) was calculated daily for the following 7 days, then the animals were killed and the brains harvested for histopathological analyses. RESULTS: All animals survived. Control rats had severe neurological dysfunction, while argon-treated animals showed significant improvements in the NDS at all time points. This was paralleled by a significant reduction in the neuronal damage index in the neocortex and the hippocampal CA 3/4 region. CONCLUSIONS: Our study demonstrates that a single 1 h application of 70% argon significantly reduced histopathological damage of the neocortex and hippocampus, associated with a marked improvement in functional neurological recovery.
BACKGROUND:Xenon has profound neuroprotective effects after neurological injury and is currently undergoing phase 2 clinical trials in cardiac arrestpatients. However, xenon is very costly, which might preclude its widespread use. We hypothesized argon, which is more available, might also protect central nervous tissues and allow better functional recovery in a rodent model of global cerebral ischaemia. METHODS: Fourteen male Sprague-Dawley rats were subjected to 7 min of cardiac arrest and 3 min of cardiopulmonary resuscitation (CPR). One hour after successful CPR, animals were randomized to either ventilation with 70% argon in oxygen (n = 7) for 1 h or 70% nitrogen (controls, n=7). A neurological deficit score (NDS) was calculated daily for the following 7 days, then the animals were killed and the brains harvested for histopathological analyses. RESULTS: All animals survived. Control rats had severe neurological dysfunction, while argon-treated animals showed significant improvements in the NDS at all time points. This was paralleled by a significant reduction in the neuronal damage index in the neocortex and the hippocampal CA 3/4 region. CONCLUSIONS: Our study demonstrates that a single 1 h application of 70% argon significantly reduced histopathological damage of the neocortex and hippocampus, associated with a marked improvement in functional neurological recovery.
Authors: Anne Brücken; Pinar Kurnaz; Christian Bleilevens; Matthias Derwall; Joachim Weis; Kay Nolte; Rolf Rossaint; Michael Fries Journal: Neurocrit Care Date: 2015-02 Impact factor: 3.210
Authors: Rajarshi Chattaraj; Misun Hwang; Serge D Zemerov; Ivan J Dmochowski; Daniel A Hammer; Daeyeon Lee; Chandra M Sehgal Journal: Adv Healthc Mater Date: 2020-03-24 Impact factor: 9.933
Authors: Peter J Kudenchuk; Claudio Sandroni; Hendrik R Drinhaus; Bernd W Böttiger; Alain Cariou; Kjetil Sunde; Martin Dworschak; Fabio Silvio Taccone; Nicolas Deye; Hans Friberg; Steven Laureys; Didier Ledoux; Mauro Oddo; Stéphane Legriel; Philippe Hantson; Jean-Luc Diehl; Pierre-Francois Laterre Journal: Ann Intensive Care Date: 2015-09-17 Impact factor: 6.925