Literature DB >> 2338715

Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody.

S J Davis1, R L Brady, A N Barclay, K Harlos, G G Dodson, A F Williams.   

Abstract

The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3.5 A and have the orthorhombic space group P2(1)2(1)2 or P2(1)2(1)2(1). The unit cell has dimensions a = 317 A, b = 161 A and c = 41.8 A and the asymmetric unit consists of two CD4:Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.

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Year:  1990        PMID： 2338715     DOI： 10.1016/S0022-2836(05)80116-0

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  5 in total

1.  Crystal structure of an HIV-binding recombinant fragment of human CD4.

Authors:  S E Ryu; P D Kwong; A Truneh; T G Porter; J Arthos; M Rosenberg; X P Dai; N H Xuong; R Axel; R W Sweet
Journal:  Nature       Date:  1990-11-29       Impact factor: 49.962

2.  Molecular characteristics of recombinant human CD4 as deduced from polymorphic crystals.

Authors:  P D Kwong; S E Ryu; W A Hendrickson; R Axel; R M Sweet; G Folena-Wasserman; P Hensley; R W Sweet
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

3.  Role of CD4 epitopes outside the gp120-binding site during entry of human immunodeficiency virus type 1.

Authors:  J H Simon; P Stumbles; N Signoret; C Somoza; M Puklavec; Q J Sattentau; A N Barclay; W James
Journal:  J Virol       Date:  1997-02       Impact factor: 5.103

Review 4.  The human immunodeficiency virus type 1 (HIV-1) CD4 receptor and its central role in promotion of HIV-1 infection.

Authors:  S Bour; R Geleziunas; M A Wainberg
Journal:  Microbiol Rev       Date:  1995-03

5.  The dimensions of the T lymphocyte glycoprotein leukosialin and identification of linear protein epitopes that can be modified by glycosylation.

Authors:  J G Cyster; D M Shotton; A F Williams
Journal:  EMBO J       Date:  1991-04       Impact factor: 11.598
  5 in total

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