Increased numbers of extra-adrenal chromaffin cells in the abdominal paraganglia of senescent F344 rats: a possible role for the glucocorticoid receptor.

The increase in numbers of extra-adrenal chromaffin cells of abdominal paraganglia in senescent F344 rats was investigated by 5-bromo-2'-deoxyuridine immunocytochemistry. A monoclonal antibody raised against 5-bromo-2'-deoxyuridine was used to react with tissue-sections of paraganglia taken from 28-month-old animals given weekly injections of the thymidine analog over a 14-week period. No immunoreactivity was detected in the extra-adrenal chromaffin cells, whereas control sections of intestinal epithelium showed abundant immunoreactivity. Also, the profile for immunoreactivity of the glucocorticoid receptor in relation to age was compared between extra-adrenal and adrenal chromaffin cells, which share cytological characteristics, but not the increase associated with senescence. In the extra-adrenal chromaffin cells, the intensity of receptor immunostaining was unchanged, while in the adrenal chromaffin cells it decreased with age. These results indicate that hypertrophy of the paraganglia in aged F344 rats is not due to the proliferation of extra-adrenal chromaffin cells. Instead, they suggest that the chromaffin cell phenotype may be induced in pre-existing cells and that the expression of the glucocorticoid receptor has an intrinsic role in this change.