BACKGROUND: Sirolimus-based regimens have recently been introduced as nephron-sparing strategies to avoid the calcineurin inhibitors-induced nephrotoxicity. METHODS: To investigate the different effects of these 2 immunosuppressants on CD4(+)CD25(hi)FoxP3(+) regulatory T cells (Tregs) and the newly defined subsets of FoxP3(+) T cells, including CD45RA(-)FoxP3(lo) cytokine-secreting T cells (csT cells), CD45RA(-)FoxP3(hi) activated Treg (aTregs), and CD45RA(+)FoxP3(+) resting Treg (rTregs), 52 cases of renal transplant recipients who have received a maintenance immunosuppressive regimen comprised either cyclosporine (n = 34) or sirolimus (n = 18) were collected and proportion of Tregs and each subset of FoxP3(+) T cells in peripheral blood was detected by flowcytometry. RESULTS: Sirolimus significantly prompted the proportion of Tregs (5.92 ± 0.40 vs 2.19 ± 0.18; P < .001) and both of the CD45RA-negative subsets, including csT cells (4.92 ± 0.50 vs 1.83 ± 0.18; P < .001) and aTregs (1.04 ± 0.15 vs 0.23 ± 0.05; P < .05) when compared with cyclosporine. However, the rTregs were not remarkably affected (0.36 ± 0.09 vs 0.79 ± 0.28; P = .063). CONCLUSION: Sirolimus plays its immune regulatory role in renal transplantation partly by increasing the proportion of Tregs. However, the increasing of both pro- and anti-inflammatory subsets of FoxP3(+) T cells also indicates the potential compromising of the effects of sirolimus on immune regulation.
BACKGROUND:Sirolimus-based regimens have recently been introduced as nephron-sparing strategies to avoid the calcineurin inhibitors-induced nephrotoxicity. METHODS: To investigate the different effects of these 2 immunosuppressants on CD4(+)CD25(hi)FoxP3(+) regulatory T cells (Tregs) and the newly defined subsets of FoxP3(+) T cells, including CD45RA(-)FoxP3(lo) cytokine-secreting T cells (csT cells), CD45RA(-)FoxP3(hi) activated Treg (aTregs), and CD45RA(+)FoxP3(+) resting Treg (rTregs), 52 cases of renal transplant recipients who have received a maintenance immunosuppressive regimen comprised either cyclosporine (n = 34) or sirolimus (n = 18) were collected and proportion of Tregs and each subset of FoxP3(+) T cells in peripheral blood was detected by flowcytometry. RESULTS:Sirolimus significantly prompted the proportion of Tregs (5.92 ± 0.40 vs 2.19 ± 0.18; P < .001) and both of the CD45RA-negative subsets, including csT cells (4.92 ± 0.50 vs 1.83 ± 0.18; P < .001) and aTregs (1.04 ± 0.15 vs 0.23 ± 0.05; P < .05) when compared with cyclosporine. However, the rTregs were not remarkably affected (0.36 ± 0.09 vs 0.79 ± 0.28; P = .063). CONCLUSION:Sirolimus plays its immune regulatory role in renal transplantation partly by increasing the proportion of Tregs. However, the increasing of both pro- and anti-inflammatory subsets of FoxP3(+) T cells also indicates the potential compromising of the effects of sirolimus on immune regulation.
Authors: Lorenzo Gallon; Opas Traitanon; Nedjema Sustento-Reodica; Joseph Leventhal; M Javeed Ansari; Ricardo C Gehrau; Venkatesh Ariyamuthu; Sacha A De Serres; Antonio Alvarado; Darshika Chhabra; James M Mathew; Nader Najafian; Valeria Mas Journal: Kidney Int Date: 2014-10-29 Impact factor: 10.612
Authors: Maja-Theresa Dieterlen; Hartmuth B Bittner; Attila Tarnok; Jens Garbade; Stefan Dhein; Friedrich W Mohr; Markus J Barten Journal: Surg Res Pract Date: 2014-02-06
Authors: Paula Alonso-Guallart; Raimon Duran-Struuck; Jonah S Zitsman; Stephen Sameroff; Marcus Pereira; Jeffrey Stern; Erik Berglund; Nathaly Llore; Genevieve Pierre; Emily Lopes; Sigal B Kofman; Makenzie Danton; Hugo P Sondermeijer; David Woodland; Yojiro Kato; Dilrukshi K Ekanayake-Alper; Alina C Iuga; Cheng-Shie Wuu; Anette Wu; W Ian Lipkin; Rafal Tokarz; Megan Sykes; Adam Griesemer Journal: Transplantation Date: 2020-02 Impact factor: 5.385