Lin Bai1, Ning Song, Jintai Yu, Lan Tan, Yong Shen, Junxia Xie, Hong Jiang. 1. Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, Medical College of Qingdao University, Qingdao, 266071, China.
Abstract
BACKGROUND: We recently reported elevated cerebrospinal fluid (CSF) levels of soluble tumor necrosis factor (TNF) receptors and TNF-α converting enzyme (TACE) activity in subjects with Alzheimer's disease (AD) in a Caucasian population. The TNF receptor-mediated signaling pathway contributes to the production of Aβ and the subsequent cytotoxicity that is observed in AD brains. However, whether the plasma levels of soluble TNF receptors (sTNFRs) are changed is still unclear in AD patients of Han descent. METHODS: In the present study, we analyzed the plasma sTNFR levels using commercially available immunoassay kits in 76 AD patients and 40 age- and sex-matched control subjects of Northern Han Chinese descent. Furthermore, the TACE activity was measured using a solution-based assay containing a fluorescently labeled TACE substrate. RESULTS: AD patients exhibited higher levels of both sTNFR and TACE activity in their plasma compared with age- and sex-matched control subjects. The levels of sTNFR1 strongly correlated with the levels of sTNFR2 (rs =0.526, P < 0.001). In addition, the levels of sTNFR1 were significantly correlated with TACE activity (rs = 0.308, P < 0.01). CONCLUSION: These results suggest that sTNFRs and TACE activity might serve as potential peripheral diagnostic candidate biomarkers in AD.
BACKGROUND: We recently reported elevated cerebrospinal fluid (CSF) levels of soluble tumor necrosis factor (TNF) receptors and TNF-α converting enzyme (TACE) activity in subjects with Alzheimer's disease (AD) in a Caucasian population. The TNF receptor-mediated signaling pathway contributes to the production of Aβ and the subsequent cytotoxicity that is observed in AD brains. However, whether the plasma levels of soluble TNF receptors (sTNFRs) are changed is still unclear in ADpatients of Han descent. METHODS: In the present study, we analyzed the plasma sTNFR levels using commercially available immunoassay kits in 76 ADpatients and 40 age- and sex-matched control subjects of Northern Han Chinese descent. Furthermore, the TACE activity was measured using a solution-based assay containing a fluorescently labeled TACE substrate. RESULTS:ADpatients exhibited higher levels of both sTNFR and TACE activity in their plasma compared with age- and sex-matched control subjects. The levels of sTNFR1 strongly correlated with the levels of sTNFR2 (rs =0.526, P < 0.001). In addition, the levels of sTNFR1 were significantly correlated with TACE activity (rs = 0.308, P < 0.01). CONCLUSION: These results suggest that sTNFRs and TACE activity might serve as potential peripheral diagnostic candidate biomarkers in AD.
Authors: John A McIntyre; Curtis J Ramsey; Bruce D Gitter; Andrew J Saykin; Dawn R Wagenknecht; Paul A Hyslop Journal: Autoimmunity Date: 2015-02-12 Impact factor: 2.815
Authors: Maria Barberà-Cremades; Ana I Gómez; Alberto Baroja-Mazo; Laura Martínez-Alarcón; Carlos M Martínez; Carlos de Torre-Minguela; Pablo Pelegrín Journal: Front Immunol Date: 2017-07-24 Impact factor: 7.561