Literature DB >> 23363839

CHROMagar Orientation medium reduces urine culture workload.

Kanchana Manickam1, James A Karlowsky, Heather Adam, Philippe R S Lagacé-Wiens, Assunta Rendina, Paulette Pang, Brenda-Lee Murray, Michelle J Alfa.   

Abstract

Microbiology laboratories continually strive to streamline and improve their urine culture algorithms because of the high volumes of urine specimens they receive and the modest numbers of those specimens that are ultimately considered clinically significant. In the current study, we quantitatively measured the impact of the introduction of CHROMagar Orientation (CO) medium into routine use in two hospital laboratories and compared it to conventional culture on blood and MacConkey agars. Based on data extracted from our Laboratory Information System from 2006 to 2011, the use of CO medium resulted in a 28% reduction in workload for additional procedures such as Gram stains, subcultures, identification panels, agglutination tests, and biochemical tests. The average number of workload units (one workload unit equals 1 min of hands-on labor) per urine specimen was significantly reduced (P < 0.0001; 95% confidence interval [CI], 0.5326 to 1.047) from 2.67 in 2006 (preimplementation of CO medium) to 1.88 in 2011 (postimplementation of CO medium). We conclude that the use of CO medium streamlined the urine culture process and increased bench throughput by reducing both workload and turnaround time in our laboratories.

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Year:  2013        PMID: 23363839      PMCID: PMC3666797          DOI: 10.1128/JCM.02877-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  14 in total

1.  Cost-effective and rapid presumptive identification of gram-negative bacilli in routine urine, pus, and stool cultures: evaluation of the use of CHROMagar orientation medium in conjunction with simple biochemical tests.

Authors:  K Ohkusu
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

2.  Performance of four chromogenic urine culture media after one or two days of incubation compared with reference media.

Authors:  Olle Aspevall; Björn Osterman; Rakel Dittmer; Lena Stén; Emma Lindbäck; Urban Forsum
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

3.  Comparative evaluation of two commercial chromogenic media for detection and presumptive identification of urinary tract pathogens.

Authors:  C Scarparo; P Piccoli; P Ricordi; M Scagnelli
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2002-04-10       Impact factor: 3.267

4.  Practical bench comparison of BBL CHROMagar Orientation and standard two-plate media for urine cultures.

Authors:  Holly A D'Souza; Mary Campbell; Ellen Jo Baron
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948

5.  A comparison of the performance of commercially available chromogenic agars for the isolation and presumptive identification of organisms from urine.

Authors:  D Fallon; G Ackland; N Andrews; D Frodsham; S Howe; K Howells; K J Nye; R E Warren
Journal:  J Clin Pathol       Date:  2003-08       Impact factor: 3.411

Review 6.  The application of chromogenic media in clinical microbiology.

Authors:  J D Perry; A M Freydière
Journal:  J Appl Microbiol       Date:  2007-12       Impact factor: 3.772

7.  Evaluation of a new chromogenic medium for isolation and identification of common urinary tract pathogens.

Authors:  P Ciragil; M Gul; M Aral; H Ekerbicer
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2006-02       Impact factor: 3.267

8.  Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens.

Authors:  K A Hengstler; R Hammann; A M Fahr
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

9.  Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens.

Authors:  Z Samra; M Heifetz; J Talmor; E Bain; J Bahar
Journal:  J Clin Microbiol       Date:  1998-04       Impact factor: 5.948

10.  Evaluation of CHROMagar Orientation for differentiation and presumptive identification of gram-negative bacilli and Enterococcus species.

Authors:  J Merlino; S Siarakas; G J Robertson; G R Funnell; T Gottlieb; R Bradbury
Journal:  J Clin Microbiol       Date:  1996-07       Impact factor: 5.948

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  7 in total

1.  Culture of Urine Specimens by Use of chromID CPS Elite Medium Can Expedite Escherichia coli Identification and Reduce Hands-On Time in the Clinical Laboratory.

Authors:  Melanie L Yarbrough; Meghan A Wallace; Cynthia Marshall; Erin Mathias; C A Burnham
Journal:  J Clin Microbiol       Date:  2016-08-31       Impact factor: 5.948

2.  Evaluation of two chromogenic media for the isolation and identification of urinary tract pathogens.

Authors:  M Payne; D Roscoe
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-08-30       Impact factor: 3.267

3.  Direct antimicrobial susceptibility testing from positive blood culture bottles in laboratories lacking automated antimicrobial susceptibility testing systems.

Authors:  Mahadevan Kumar; S P S Shergill; Kundan Tandel; Kavita Sahai; R M Gupta
Journal:  Med J Armed Forces India       Date:  2018-12-20

4.  Evaluation of presumptive identification of Enterobacterales using CHROMagar Orientation medium and rapid biochemical tests.

Authors:  Hirofumi Ohtaki; Akifumi Takahashi; Ayumi Niwa; Jun Yonetamari; Asami Nakayama; Tomokazu Kuchibiro; Hirotoshi Ohta; Hiroyasu Ito; Hisashi Baba; Nobuo Murakami; Kiyofumi Ohkusu
Journal:  J Clin Lab Anal       Date:  2020-06-28       Impact factor: 2.352

5.  Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry for Identification of Microorganisms in Clinical Urine Specimens after Two Pretreatments.

Authors:  Deru Lei; Peiying Chen; Xueting Chen; Yujie Zong; Xiangyang Li
Journal:  Pol J Microbiol       Date:  2021

6.  Comparative evaluation of chromogenic agar medium and conventional culture system for isolation and presumptive identification of uropathogens.

Authors:  Laila Akter; Rezwana Haque; Md Abdus Salam
Journal:  Pak J Med Sci       Date:  2014-09       Impact factor: 1.088

7.  Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar™ Orientation medium.

Authors:  Atul K Singh; Arun K Bhunia
Journal:  Microb Biotechnol       Date:  2015-10-27       Impact factor: 5.813

  7 in total

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