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Literature DB >> 23360306

Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.

Matthew D Lew¹, Mikael P Backlund, W E Moerner.

Abstract

The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60° can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.

Entities: Chemical Disease Gene Species

Mesh：

Year: 2013 PMID： 23360306 PMCID： PMC3696529 DOI： 10.1021/nl304359p

Source DB: PubMed Journal: Nano Lett ISSN： 1530-6984 Impact factor: 11.189

21 in total

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40 in total

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2. Single molecule optical measurements of orientation and rotations of biological macromolecules.

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Review 3. Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.

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Journal: Chem Rev Date: 2017-02-02 Impact factor: 60.622

4. Determining the rotational mobility of a single molecule from a single image: a numerical study.

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Journal: Opt Express Date: 2015-02-23 Impact factor: 3.894

5. Localization accuracy of gold nanoparticles in single particle orientation and rotational tracking.

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6. Imaging the three-dimensional orientation and rotational mobility of fluorescent emitters using the Tri-spot point spread function.

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7. Polarization of excitation light influences molecule counting in single-molecule localization microscopy.

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9. A bisected pupil for studying single-molecule orientational dynamics and its application to three-dimensional super-resolution microscopy.

Authors: Adam S Backer; Mikael P Backlund; Alexander R von Diezmann; Steffen J Sahl; W E Moerner
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10. The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation.

Authors: Mikael P Backlund; Matthew D Lew; Adam S Backer; Steffen J Sahl; Ginni Grover; Anurag Agrawal; Rafael Piestun; W E Moerner
Journal: Proc SPIE Int Soc Opt Eng Date: 2013-02-22