Literature DB >> 2334945

Optimization of cRNA probe in situ hybridization methodology for localization of glucocorticoid receptor mRNA in rat brain: a detailed protocol.

H J Whitfield1, L S Brady, M A Smith, E Mamalaki, R J Fox, M Herkenham.   

Abstract

1. We have described a general ribonucleotide probe in situ hybridization methodology for localization of mRNA in frozen, unfixed tissue sections of brain. 2. The most important steps in obtaining consistent and reproducible autoradiographs with ribonucleotide probes were tissue acetylation and application of the radiolabeled probe to tissue sections under unsealed, glass coverslips. 3. Variability of the hybridization signal in tissue sections has been minimized to achieve a high degree of reproducibility within a given experiment as determined by densitometric analysis of rat glucocorticoid and mineralocorticoid receptor mRNA hybridization autoradiographs. 4. Tissue quality has been optimized for high-resolution anatomical localization of mRNA species by nuclear track emulsion. 5. The protocol is amenable to rapid, batchwise processing of tissue samples.

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Year:  1990        PMID： 2334945     DOI： 10.1007/bf00733641

Source DB:  PubMed          Journal:  Cell Mol Neurobiol        ISSN： 0272-4340            Impact factor:   5.046


  13 in total

1.  Detection of viral sequences of low reiteration frequency by in situ hybridization.

Authors:  M Brahic; A T Haase
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

2.  Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

Authors:  S Hayashi; I C Gillam; A D Delaney; G M Tener
Journal:  J Histochem Cytochem       Date:  1978-08       Impact factor: 2.479

3.  In situ hybridization histochemical detection of neuropeptide mRNA using DNA and RNA probes.

Authors:  W S Young
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

4.  Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA.

Authors:  R Miesfeld; S Rusconi; P J Godowski; B A Maler; S Okret; A C Wikström; J A Gustafsson; K R Yamamoto
Journal:  Cell       Date:  1986-08-01       Impact factor: 41.582

Review 5.  Adrenal steroid receptors and actions in the nervous system.

Authors:  B S McEwen; E R De Kloet; W Rostene
Journal:  Physiol Rev       Date:  1986-10       Impact factor: 37.312

6.  Localization of glucocorticoid receptor mRNA in the male rat brain by in situ hybridization.

Authors:  M Aronsson; K Fuxe; Y Dong; L F Agnati; S Okret; J A Gustafsson
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

7.  Binding of radiolabeled opiates to slide-mounted sections of molded minced rat brain: a novel method for conducting radioreceptor assays.

Authors:  R B Rothman; U K Schumacher; C B Pert
Journal:  Neuropeptides       Date:  1983-10       Impact factor: 3.286

8.  Localization of glucocorticoid receptor messenger ribonucleic acid in hippocampus of rat brain using in situ hybridization.

Authors:  G Yang; M F Matocha; S I Rapoport
Journal:  Mol Endocrinol       Date:  1988-08

9.  Distribution of the mineralocorticoid and the glucocorticoid receptor mRNAs in the rat hippocampus.

Authors:  J A Van Eekelen; W Jiang; E R De Kloet; M C Bohn
Journal:  J Neurosci Res       Date:  1988-09       Impact factor: 4.164

10.  In situ hybridization mapping of glucocorticoid receptor messenger ribonucleic acid in rat brain.

Authors:  R J Sousa; N H Tannery; E M Lafer
Journal:  Mol Endocrinol       Date:  1989-03
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  38 in total

1.  Characteristics of C6 glioma cells overexpressing a gap junction protein.

Authors:  C C Naus; D Zhu; S D Todd; G M Kidder
Journal:  Cell Mol Neurobiol       Date:  1992-04       Impact factor: 5.046

2.  Increased interstitial white matter neuron density in the dorsolateral prefrontal cortex of people with schizophrenia.

Authors:  Yang Yang; Samantha J Fung; Alice Rothwell; Si Tianmei; Cynthia Shannon Weickert
Journal:  Biol Psychiatry       Date:  2010-10-25       Impact factor: 13.382

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Authors:  Cornelia Voigt; Stefan Leitner; Manfred Gahr
Journal:  Proc Biol Sci       Date:  2007-11-07       Impact factor: 5.349

4.  Sex difference in the size of the neural song control regions in a dueting songbird with similar song repertoire size of males and females.

Authors:  M Gahr; E Sonnenschein; W Wickler
Journal:  J Neurosci       Date:  1998-02-01       Impact factor: 6.167

5.  Neuroendocrine correlates of sex-role reversal in barred buttonquails.

Authors:  Cornelia Voigt
Journal:  Proc Biol Sci       Date:  2016-11-30       Impact factor: 5.349

6.  Metabotropic glutamate receptor 2 and 3 gene expression in the human prefrontal cortex and mesencephalon in schizophrenia.

Authors:  Subroto Ghose; Jeremy M Crook; Cynthia L Bartus; Thomas G Sherman; Mary M Herman; Thomas M Hyde; Joel E Kleinman; Mayada Akil
Journal:  Int J Neurosci       Date:  2008-11       Impact factor: 2.292

7.  Generation and behavioral characterization of beta-catenin forebrain-specific conditional knock-out mice.

Authors:  Todd D Gould; Kelley C O'Donnell; Alyssa M Picchini; Eliot R Dow; Guang Chen; Husseini K Manji
Journal:  Behav Brain Res       Date:  2008-01-12       Impact factor: 3.332

8.  Gonadal steroids exert facilitating and "buffering" effects on glucocorticoid-mediated transcriptional regulation of corticotropin-releasing hormone and corticosteroid receptor genes in rat brain.

Authors:  V K Patchev; O F Almeida
Journal:  J Neurosci       Date:  1996-11-01       Impact factor: 6.167

9.  Severity of neurological signs and degree of inflammatory lesions in the brains of rats with Borna disease correlate with the induction of nitric oxide synthase.

Authors:  Y M Zheng; M K Schäfer; E Weihe; H Sheng; S Corisdeo; Z F Fu; H Koprowski; B Dietzschold
Journal:  J Virol       Date:  1993-10       Impact factor: 5.103

10.  Hippocampal glucocorticoid receptor expression in the tree shrew: regulation by psychosocial conflict.

Authors:  O Jöhren; G Flügge; E Fuchs
Journal:  Cell Mol Neurobiol       Date:  1994-06       Impact factor: 5.046
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