PURPOSE: To develop a tissue fixation method that preserves in vivo manganese enhancement for ex vivo magnetic resonance imaging (MRI). The needs are clear, as conventional in vivo manganese-enhanced MRI (MEMRI) applied to live animals is time-limited, hence limited in spatial resolution and signal-to-noise ratio (SNR). Ex vivo applications can achieve superior spatial resolution and SNR through increased signal averaging and optimized radiofrequency coil designs. A tissue fixation method that preserves in vivo Mn(2+) enhancement postmortem is necessary for ex vivo MEMRI. MATERIALS AND METHODS: T1 measurements and T1 -weighted MRI were performed on MnCl2 -administered mice. The mice were then euthanized and the brains were fixed using one of two brain tissue fixation methods: aldehyde solution or focused beam microwave irradiation (FBMI). MRI was then performed on the fixed brains. RESULTS: T1 values and T1 -weighted signal contrasts were comparable between in vivo and ex vivo scans on aldehyde-fixed brains. FBMI resulted in the loss of Mn(2+) enhancement. CONCLUSION: Aldehyde fixation, not FBMI, maintained in vivo manganese enhancement for ex vivo MEMRI.
PURPOSE: To develop a tissue fixation method that preserves in vivo manganese enhancement for ex vivo magnetic resonance imaging (MRI). The needs are clear, as conventional in vivo manganese-enhanced MRI (MEMRI) applied to live animals is time-limited, hence limited in spatial resolution and signal-to-noise ratio (SNR). Ex vivo applications can achieve superior spatial resolution and SNR through increased signal averaging and optimized radiofrequency coil designs. A tissue fixation method that preserves in vivo Mn(2+) enhancement postmortem is necessary for ex vivo MEMRI. MATERIALS AND METHODS: T1 measurements and T1 -weighted MRI were performed on MnCl2 -administered mice. The mice were then euthanized and the brains were fixed using one of two brain tissue fixation methods: aldehyde solution or focused beam microwave irradiation (FBMI). MRI was then performed on the fixed brains. RESULTS: T1 values and T1 -weighted signal contrasts were comparable between in vivo and ex vivo scans on aldehyde-fixed brains. FBMI resulted in the loss of Mn(2+) enhancement. CONCLUSION:Aldehyde fixation, not FBMI, maintained in vivo manganese enhancement for ex vivo MEMRI.
Authors: Robin A de Graaf; Golam M I Chowdhury; Peter B Brown; Douglas L Rothman; Kevin L Behar Journal: J Neurochem Date: 2009-02-07 Impact factor: 5.372
Authors: M M Sperry; B M Kandel; S Wehrli; K N Bass; S R Das; P S Dhillon; J C Gee; G A Barr Journal: Neuroscience Date: 2017-04-06 Impact factor: 3.590
Authors: Holly E Holmes; Nick M Powell; Da Ma; Ozama Ismail; Ian F Harrison; Jack A Wells; Niall Colgan; James M O'Callaghan; Ross A Johnson; Tracey K Murray; Zeshan Ahmed; Morten Heggenes; Alice Fisher; M Jorge Cardoso; Marc Modat; Michael J O'Neill; Emily C Collins; Elizabeth M C Fisher; Sébastien Ourselin; Mark F Lythgoe Journal: Front Neuroinform Date: 2017-03-31 Impact factor: 4.081