Literature DB >> 23345893

Determination of some hydrodynamic parameters of ovine serum albumin solutions using viscometric measurements.

Karol Monkos1.   

Abstract

The influence of protein concentration and temperature on the viscosity of ovine serum albumin (OSA) solutions was studied. The Mooney equation and a modified Arrhenius formula were used to described the viscosity-concentration and viscosity-temperature dependence of the solutions, respectively. The effective specific volume, the activation energy and entropy of viscous flow for hydrated OSA were calculated. The axial ratio and the dimensions of the main semi-axes of hydrated OSA were established. At low concentration limit, the temperature dependence of the intrinsic viscosity and Huggins coefficient is presented. Comparison of some hydrodynamic parameters obtained for different proteins has been made.

Entities:  

Keywords:  hydrodynamic parameters; ovine serum albumin; viscosity

Year:  2005        PMID: 23345893      PMCID: PMC3456155          DOI: 10.1007/s10867-005-1830-z

Source DB:  PubMed          Journal:  J Biol Phys        ISSN: 0092-0606            Impact factor:   1.365


  21 in total

1.  A comparative study on viscosity of human, bovine and pig IgG immunoglobulins in aqueous solutions.

Authors:  K Monkos; B Turczynski
Journal:  Int J Biol Macromol       Date:  1999-11       Impact factor: 6.953

2.  The three recombinant domains of human serum albumin. Structural characterization and ligand binding properties.

Authors:  M Dockal; D C Carter; F Rüker
Journal:  J Biol Chem       Date:  1999-10-08       Impact factor: 5.157

3.  The structure and dipole moment of globular proteins in solution and crystalline states: use of NMR and X-ray databases for the numerical calculation of dipole moment.

Authors:  S Takashima
Journal:  Biopolymers       Date:  2001-04-05       Impact factor: 2.505

4.  Generalized concentration dependence of globular protein self-diffusion coefficients in aqueous solutions.

Authors:  Irina V Nesmelova; Vladimir D Skirda; Vladimir D Fedotov
Journal:  Biopolymers       Date:  2002-02       Impact factor: 2.505

5.  On the hydrodynamics and temperature dependence of the solution conformation of human serum albumin from viscometry approach.

Authors:  Karol Monkos
Journal:  Biochim Biophys Acta       Date:  2004-07-01

6.  Determination of the axial ratio of globular proteins in aqueous solution using viscometric measurements.

Authors:  K Monkos; B Turczynski
Journal:  Int J Biol Macromol       Date:  1991-12       Impact factor: 6.953

Review 7.  The intrinsic viscosity of biological macromolecules. Progress in measurement, interpretation and application to structure in dilute solution.

Authors:  S E Harding
Journal:  Prog Biophys Mol Biol       Date:  1997       Impact factor: 3.667

8.  Interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants: spectroscopy and modelling.

Authors:  E L Gelamo; C H T P Silva; H Imasato; M Tabak
Journal:  Biochim Biophys Acta       Date:  2002-01-31

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Authors:  T Peters
Journal:  Adv Protein Chem       Date:  1985

10.  X-ray and primary structure of horse serum albumin (Equus caballus) at 0.27-nm resolution.

Authors:  J X Ho; E W Holowachuk; E J Norton; P D Twigg; D C Carter
Journal:  Eur J Biochem       Date:  1993-07-01
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