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Literature DB >> 23345287

Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens.

Gavin A Cloherty¹, James Rhoads, Thomas P Young, Neil T Parkin, Vera Holzmayer, Lilly Yuen, Carolyn Mullen.

Abstract

The Abbott RealTime HBV assay targets the N-terminal region of the S gene. Here we analyzed the sequence variability of the assay target region from >2,100 clinical specimens. Thermodynamic modeling of the percentage of bound primer/probe at the assay annealing temperature was performed to assess the potential effect of sequence variability.

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Year: 2013 PMID： 23345287 PMCID： PMC3666792 DOI： 10.1128/JCM.03003-12

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

24 in total

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Review 8. Hepatitis B virus genetic diversity and its impact on diagnostic assays.

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9. Clinical and resistance consequences of misquantification of plasma and cerebrospinal fluid human immunodeficiency virus type 1 (HIV-1) RNA in samples from an HIV-1 subtype G-infected patient.

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