| Literature DB >> 23341627 |
Kousuke Hanada1, Mieko Higuchi-Takeuchi, Masanori Okamoto, Takeshi Yoshizumi, Minami Shimizu, Kentaro Nakaminami, Ranko Nishi, Chihiro Ohashi, Kei Iida, Maho Tanaka, Yoko Horii, Mika Kawashima, Keiko Matsui, Tetsuro Toyoda, Kazuo Shinozaki, Motoaki Seki, Minami Matsui.
Abstract
It is likely that many small ORFs (sORFs; 30-100 amino acids) are missed when genomes are annotated. To overcome this limitation, we identified ∼8,000 sORFs with high coding potential in intergenic regions of the Arabidopsis thaliana genome. However, the question remains as to whether these coding sORFs play functional roles. Using a designed array, we generated an expression atlas for 16 organs and 17 environmental conditions among 7,901 identified coding sORFs. A total of 2,099 coding sORFs were highly expressed under at least one experimental condition, and 571 were significantly conserved in other land plants. A total of 473 coding sORFs were overexpressed; ∼10% (49/473) induced visible phenotypic effects, a proportion that is approximately seven times higher than that of randomly chosen known genes. These results indicate that many coding sORFs hidden in plant genomes are associated with morphogenesis. We believe that the expression atlas will contribute to further study of the roles of sORFs in plants.Entities:
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Year: 2013 PMID： 23341627 PMCID： PMC3568369 DOI： 10.1073/pnas.1213958110
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205