Literature DB >> 23335760

Directed construction and analysis of a Sinorhizobium meliloti pSymA deletion mutant library.

Svetlana N Yurgel1, Michael W Mortimer, Jennifer T Rice, Jodi L Humann, Michael L Kahn.   

Abstract

Resources from the Sinorhizobium meliloti Rm1021 open reading frame (ORF) plasmid libraries were used in a medium-throughput method to construct a set of 50 overlapping deletion mutants covering all of the Rm1021 pSymA megaplasmid except the replicon region. Each resulting pSymA derivative carried a defined deletion of approximately 25 ORFs. Various phenotypes, including cytochrome c respiration activity, the ability of the mutants to grow on various carbon and nitrogen sources, and the symbiotic effectiveness of the mutants with alfalfa, were analyzed. This approach allowed us to systematically evaluate the potential impact of regions of Rm1021 pSymA for their free-living and symbiotic phenotypes.

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Year:  2013        PMID： 23335760      PMCID： PMC3592244          DOI： 10.1128/AEM.02974-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  37 in total

1.  Megaplasmid pRme2011a of Sinorhizobium meliloti is not required for viability.

Authors:  I J Oresnik; S L Liu; C K Yost; M F Hynes
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

2.  Characterization of the twin-arginine transport secretome in Sinorhizobium meliloti and evidence for host-dependent phenotypes.

Authors:  Brad S Pickering; Harry Yudistira; Ivan J Oresnik
Journal:  Appl Environ Microbiol       Date:  2012-07-27       Impact factor: 4.792

3.  Genetic system for reversible integration of DNA constructs and lacZ gene fusions into the Escherichia coli chromosome.

Authors:  R Platt; C Drescher; S K Park; G J Phillips
Journal:  Plasmid       Date:  2000-01       Impact factor: 3.466

4.  The composite genome of the legume symbiont Sinorhizobium meliloti.

Authors:  F Galibert; T M Finan; S R Long; A Puhler; P Abola; F Ampe; F Barloy-Hubler; M J Barnett; A Becker; P Boistard; G Bothe; M Boutry; L Bowser; J Buhrmester; E Cadieu; D Capela; P Chain; A Cowie; R W Davis; S Dreano; N A Federspiel; R F Fisher; S Gloux; T Godrie; A Goffeau; B Golding; J Gouzy; M Gurjal; I Hernandez-Lucas; A Hong; L Huizar; R W Hyman; T Jones; D Kahn; M L Kahn; S Kalman; D H Keating; E Kiss; C Komp; V Lelaure; D Masuy; C Palm; M C Peck; T M Pohl; D Portetelle; B Purnelle; U Ramsperger; R Surzycki; P Thebault; M Vandenbol; F J Vorholter; S Weidner; D H Wells; K Wong; K C Yeh; J Batut
Journal:  Science       Date:  2001-07-27       Impact factor: 47.728

5.  Genetic characterization of a complex locus necessary for the transport and catabolism of erythritol, adonitol and L-arabitol in Sinorhizobium meliloti.

Authors:  Barney A Geddes; Ivan J Oresnik
Journal:  Microbiology (Reading)       Date:  2012-05-18       Impact factor: 2.777

6.  Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid.

Authors:  M J Barnett; R F Fisher; T Jones; C Komp; A P Abola; F Barloy-Hubler; L Bowser; D Capela; F Galibert; J Gouzy; M Gurjal; A Hong; L Huizar; R W Hyman; D Kahn; M L Kahn; S Kalman; D H Keating; C Palm; M C Peck; R Surzycki; D H Wells; K C Yeh; R W Davis; N A Federspiel; S R Long
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-31       Impact factor: 11.205

7.  Role of potassium uptake systems in Sinorhizobium meliloti osmoadaptation and symbiotic performance.

Authors:  Ana Domínguez-Ferreras; Socorro Muñoz; José Olivares; María J Soto; Juan Sanjuán
Journal:  J Bacteriol       Date:  2009-01-30       Impact factor: 3.490

8.  Construction and expression of sugar kinase transcriptional gene fusions by using the Sinorhizobium meliloti ORFeome.

Authors:  Jodi L Humann; Brenda K Schroeder; Michael W Mortimer; Brent L House; Svetlana N Yurgel; Scott C Maloney; Kristel L Ward; Heather M Fallquist; Hope T Ziemkiewicz; Michael L Kahn
Journal:  Appl Environ Microbiol       Date:  2008-09-12       Impact factor: 4.792

9.  The GntR-type regulators gtrA and gtrB affect cell growth and nodulation of Sinorhizobium meliloti.

Authors:  Yi Wang; Ai-Min Chen; Ai-Yuan Yu; Li Luo; Guan-Qian Yu; Jia-Bi Zhu; Yan-Zhang Wang
Journal:  J Microbiol       Date:  2008-06-11       Impact factor: 3.422

10.  Genetic organization of the region encoding regulation, biosynthesis, and transport of rhizobactin 1021, a siderophore produced by Sinorhizobium meliloti.

Authors:  D Lynch; J O'Brien; T Welch; P Clarke; P O Cuív; J H Crosa; M O'Connell
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490
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  4 in total

1.  Cell growth inhibition upon deletion of four toxin-antitoxin loci from the megaplasmids of Sinorhizobium meliloti.

Authors:  Branislava Milunovic; George C diCenzo; Richard A Morton; Turlough M Finan
Journal:  J Bacteriol       Date:  2013-12-06       Impact factor: 3.490

2.  Examination of prokaryotic multipartite genome evolution through experimental genome reduction.

Authors:  George C diCenzo; Allyson M MacLean; Branislava Milunovic; G Brian Golding; Turlough M Finan
Journal:  PLoS Genet       Date:  2014-10-23       Impact factor: 5.917

3.  Functional relationships between plasmids and their significance for metabolism and symbiotic performance of Rhizobium leguminosarum bv. trifolii.

Authors:  Grażyna Stasiak; Andrzej Mazur; Jerzy Wielbo; Małgorzata Marczak; Kamil Zebracki; Piotr Koper; Anna Skorupska
Journal:  J Appl Genet       Date:  2014-05-17       Impact factor: 3.240

4.  Metabolic modelling reveals the specialization of secondary replicons for niche adaptation in Sinorhizobium meliloti.

Authors:  George C diCenzo; Alice Checcucci; Marco Bazzicalupo; Alessio Mengoni; Carlo Viti; Lukasz Dziewit; Turlough M Finan; Marco Galardini; Marco Fondi
Journal:  Nat Commun       Date:  2016-07-22       Impact factor: 14.919
  4 in total

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