PURPOSE: The purpose of this study was to determine the influence of Maillard reaction (MR, glycation) on biochemical and biological properties of the major peanut allergen Ara h 1. METHODS: Three different time/temperature conditions of treatment were applied (37, 60, and 145 °C). The extent of MR was assessed by SDS-PAGE, loss of free amino groups, fluorescence intensity, content of bound sugar and fructosamine. The Caco-2 model system was applied to study effects of hydrolysed and non-hydrolysed Ara h 1 on proliferation and interleukin-8 (IL-8) secretion from Caco-2 cells. RESULTS: We demonstrated significant differences in the biochemical properties of Ara h 1 glycated at different time/temperature conditions. Glycation of Ara h 1 at 37 °C was shown to cause least biochemical changes, not limiting pepsin hydrolysis. Loss of free amino groups, increase of fluorescence and brown colour of Ara h 1 glycated at 60 and 145 °C indicated advanced and final stages of MR. Non-treated Ara h 1 inhibited Caco-2 cell proliferation and stimulated IL-8 secretion. This effect was less pronounced for glycated Ara h 1. Incubation of Caco-2 cells with non-hydrolysed Ara h 1, glycated at the temperature of 37 and 60 °C, did not stimulate IL-8 secretion. CONCLUSION: Each applied time/temperature-treatment combination caused different biochemical changes of Ara h 1, underlining diversity of formed MRPs. MR, independently of temperature/time conditions, reduced the pro-inflammatory properties of native Ara h 1, reflected in stimulation of IL-8 secretion from intestinal epithelial cells.
PURPOSE: The purpose of this study was to determine the influence of Maillard reaction (MR, glycation) on biochemical and biological properties of the major peanut allergen Ara h 1. METHODS: Three different time/temperature conditions of treatment were applied (37, 60, and 145 °C). The extent of MR was assessed by SDS-PAGE, loss of free amino groups, fluorescence intensity, content of bound sugar and fructosamine. The Caco-2 model system was applied to study effects of hydrolysed and non-hydrolysed Ara h 1 on proliferation and interleukin-8 (IL-8) secretion from Caco-2 cells. RESULTS: We demonstrated significant differences in the biochemical properties of Ara h 1 glycated at different time/temperature conditions. Glycation of Ara h 1 at 37 °C was shown to cause least biochemical changes, not limiting pepsin hydrolysis. Loss of free amino groups, increase of fluorescence and brown colour of Ara h 1 glycated at 60 and 145 °C indicated advanced and final stages of MR. Non-treated Ara h 1 inhibited Caco-2 cell proliferation and stimulated IL-8 secretion. This effect was less pronounced for glycated Ara h 1. Incubation of Caco-2 cells with non-hydrolysed Ara h 1, glycated at the temperature of 37 and 60 °C, did not stimulate IL-8 secretion. CONCLUSION: Each applied time/temperature-treatment combination caused different biochemical changes of Ara h 1, underlining diversity of formed MRPs. MR, independently of temperature/time conditions, reduced the pro-inflammatory properties of native Ara h 1, reflected in stimulation of IL-8 secretion from intestinal epithelial cells.
Authors: Y M Vissers; M Iwan; K Adel-Patient; P Stahl Skov; N M Rigby; P E Johnson; P Mandrup Müller; L Przybylski-Nicaise; M Schaap; J Ruinemans-Koerts; A P H Jansen; E N C Mills; H F J Savelkoul; H J Wichers Journal: Clin Exp Allergy Date: 2011-08-01 Impact factor: 5.018
Authors: Evelien L van Boxtel; Miranda M C van Beers; Stef J Koppelman; Lambertus A M van den Broek; Harry Gruppen Journal: J Agric Food Chem Date: 2006-09-20 Impact factor: 5.279
Authors: G A Mueller; S J Maleki; K Johnson; B K Hurlburt; H Cheng; S Ruan; J B Nesbit; A Pomés; L L Edwards; A Schorzman; L J Deterding; H Park; K B Tomer; R E London; J G Williams Journal: Allergy Date: 2013-11-23 Impact factor: 13.146
Authors: Katina L Johnson; Jason G Williams; Soheila J Maleki; Barry K Hurlburt; Robert E London; Geoffrey A Mueller Journal: J Agric Food Chem Date: 2016-02-05 Impact factor: 5.279
Authors: Joana Costa; Simona Lucia Bavaro; Sara Benedé; Araceli Diaz-Perales; Cristina Bueno-Diaz; Eva Gelencser; Julia Klueber; Colette Larré; Daniel Lozano-Ojalvo; Roberta Lupi; Isabel Mafra; Gabriel Mazzucchelli; Elena Molina; Linda Monaci; Laura Martín-Pedraza; Cristian Piras; Pedro M Rodrigues; Paola Roncada; Denise Schrama; Tanja Cirkovic-Velickovic; Kitty Verhoeckx; Caterina Villa; Annette Kuehn; Karin Hoffmann-Sommergruber; Thomas Holzhauser Journal: Clin Rev Allergy Immunol Date: 2022-02 Impact factor: 8.667
Authors: Gemma González-Ortiz; Sílvia Bronsoms; H C Quarles Van Ufford; S Bart A Halkes; Ritva Virkola; Rob M J Liskamp; Cees J Beukelman; Roland J Pieters; José Francisco Pérez; Susana María Martín-Orúe Journal: PLoS One Date: 2014-08-13 Impact factor: 3.240