| Literature DB >> 23334020 |
Fengcheng Li1, Shuangfeng Ren, Wei Zhang, Zhengdan Xu, Guosheng Xie, Yan Chen, Yuanyuan Tu, Qing Li, Shiguang Zhou, Yu Li, Fen Tu, Lin Liu, Yanting Wang, Jianxiong Jiang, Jingping Qin, Shizhong Li, Qiwei Li, Hai-Chun Jing, Fasong Zhou, Neal Gutterson, Liangcai Peng.
Abstract
Xylans are the major hemicelluloses in grasses, but their effects on biomass saccharification remain unclear. In this study, we examined the 79 representative Miscanthus accessions that displayed a diverse cell wall composition and varied biomass digestibility. Correlation analysis showed that hemicelluloses level has a strong positive effect on lignocellulose enzymatic digestion after NaOH or H(2)SO(4) pretreatment. Characterization of the monosaccharide compositions in the KOH-extractable and non-KOH-extractable hemicelluloses indicated that arabinose substitution degree of xylan is the key factor that positively affects biomass saccharification. The xylose/arabinose ratio after individual enzyme digestion revealed that the arabinose in xylan is partially associated with cellulose in the amorphous regions, which negatively affects cellulose crystallinity for high biomass digestibility. The results provide insights into the mechanism of lignocellulose enzymatic digestion upon pretreatment, and also suggest a goal for the genetic modification of hemicelluloses towards the bioenergy crop breeding of Miscanthus and grasses.Entities:
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Year: 2012 PMID： 23334020 DOI： 10.1016/j.biortech.2012.12.107
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642