Literature DB >> 23333644

Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.

Takashi Yamano1, Hiro Iguchi, Hideya Fukuzawa.   

Abstract

Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions.
Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

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Year:  2013        PMID： 23333644     DOI： 10.1016/j.jbiosc.2012.12.020

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  44 in total

1.  Origins of multicellular complexity: Volvox and the volvocine algae.

Authors:  Matthew D Herron
Journal:  Mol Ecol       Date:  2016-03-01       Impact factor: 6.185

2.  Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii.

Authors:  Lianyong Wang; Takashi Yamano; Masataka Kajikawa; Masafumi Hirono; Hideya Fukuzawa
Journal:  Photosynth Res       Date:  2014-02-19       Impact factor: 3.573

3.  The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response.

Authors:  Karina Perlaza; Hannah Toutkoushian; Morgane Boone; Mable Lam; Masakazu Iwai; Martin C Jonikas; Peter Walter; Silvia Ramundo
Journal:  Elife       Date:  2019-10-15       Impact factor: 8.140

4.  In vivo system for analyzing the function of the PsbP protein using Chlamydomonas reinhardtii.

Authors:  Taishi Nishimura; Fumihiko Sato; Kentaro Ifuku
Journal:  Photosynth Res       Date:  2017-03-24       Impact factor: 3.573

5.  RNA-binding protein DUS16 plays an essential role in primary miRNA processing in the unicellular alga Chlamydomonas reinhardtii.

Authors:  Tomohito Yamasaki; Masayuki Onishi; Eun-Jeong Kim; Heriberto Cerutti; Takeshi Ohama
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-31       Impact factor: 11.205

6.  Detailed identification of fatty acid isomers sheds light on the probable precursors of triacylglycerol accumulation in photoautotrophically grown Chlamydomonas reinhardtii.

Authors:  Kenta Sakurai; Takashi Moriyama; Naoki Sato
Journal:  Eukaryot Cell       Date:  2013-12-13

Review 7.  A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.

Authors:  Patrice A Salomé; Sabeeha S Merchant
Journal:  Plant Cell       Date:  2019-06-12       Impact factor: 11.277

8.  Algal dual-specificity tyrosine phosphorylation-regulated kinase, triacylglycerol accumulation regulator1, regulates accumulation of triacylglycerol in nitrogen or sulfur deficiency.

Authors:  Masataka Kajikawa; Yuri Sawaragi; Haruka Shinkawa; Takashi Yamano; Akira Ando; Misako Kato; Masafumi Hirono; Naoki Sato; Hideya Fukuzawa
Journal:  Plant Physiol       Date:  2015-04-28       Impact factor: 8.340

9.  High-resolution suborganellar localization of Ca2+-binding protein CAS, a novel regulator of CO2-concentrating mechanism.

Authors:  Takashi Yamano; Chihana Toyokawa; Hideya Fukuzawa
Journal:  Protoplasma       Date:  2018-01-25       Impact factor: 3.356

10.  Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii.

Authors:  Noriko Ueki; Takahiro Ide; Shota Mochiji; Yuki Kobayashi; Ryutaro Tokutsu; Norikazu Ohnishi; Katsushi Yamaguchi; Shuji Shigenobu; Kan Tanaka; Jun Minagawa; Toru Hisabori; Masafumi Hirono; Ken-Ichi Wakabayashi
Journal:  Proc Natl Acad Sci U S A       Date:  2016-04-27       Impact factor: 11.205
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