L Yin1, F Shi, X Hu, C Chen, X Wang. 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
Abstract
AIMS: To increase the l-isoleucine production in Corynebacterium glutamicum by overexpressing the global regulator Lrp and the two-component export system BrnFE. METHODS AND RESULTS: The brnFE operon and the lrp gene were cloned into the shuttle vector pDXW-8 individually or in combination. The constructed plasmids were transformed into an l-isoleucine-producing strain C. glutamicum JHI3-156, and the l-isoleucine production in these different strains was analysed and compared. More l-isoleucine was produced when only Lrp was expressed than when only BrnFE was expressed. Significant increase in l-isoleucine production was observed when Lrp and BrnFE were expressed in combination. Compared to the control strain, l-isoleucine production in JHI3-156/pDXW-8-lrp-brnFE increased 63% in flask cultivation, and the specific yield of l-isoleucine increased 72% in fed-batch fermentation. CONCLUSIONS: Both Lrp and BrnFE are important to enhance the l-isoleucine production in C. glutamicum. SIGNIFICANCE AND IMPACT OF THE STUDY: The results provide useful information to enhance l-isoleucine or other branched-chain amino acid production in C. glutamicum.
AIMS: To increase the l-isoleucine production in Corynebacterium glutamicum by overexpressing the global regulator Lrp and the two-component export system BrnFE. METHODS AND RESULTS: The brnFE operon and the lrp gene were cloned into the shuttle vector pDXW-8 individually or in combination. The constructed plasmids were transformed into an l-isoleucine-producing strain C. glutamicum JHI3-156, and the l-isoleucine production in these different strains was analysed and compared. More l-isoleucine was produced when only Lrp was expressed than when only BrnFE was expressed. Significant increase in l-isoleucine production was observed when Lrp and BrnFE were expressed in combination. Compared to the control strain, l-isoleucine production in JHI3-156/pDXW-8-lrp-brnFE increased 63% in flask cultivation, and the specific yield of l-isoleucine increased 72% in fed-batch fermentation. CONCLUSIONS: Both Lrp and BrnFE are important to enhance the l-isoleucine production in C. glutamicum. SIGNIFICANCE AND IMPACT OF THE STUDY: The results provide useful information to enhance l-isoleucine or other branched-chain amino acid production in C. glutamicum.
Authors: Nurije Mustafi; Alexander Grünberger; Regina Mahr; Stefan Helfrich; Katharina Nöh; Bastian Blombach; Dietrich Kohlheyer; Julia Frunzke Journal: PLoS One Date: 2014-01-17 Impact factor: 3.240