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Literature DB >> 23315727

Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing.

David VanInsberghe¹, Martin Hartmann, Gordon R Stewart, William W Mohn.

Abstract

We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units.

Mesh：

Substances：
DNA, Bacterial

Year: 2013 PMID： 23315727 PMCID： PMC3592214 DOI： 10.1128/AEM.03112-12

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

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