| Literature DB >> 23313179 |
Anil Kumar Patel1, Celine Laroche, Alain Marcati, Alina Violeta Ursu, Sébastien Jubeau, Luc Marchal, Emmanuel Petit, Gholamreza Djelveh, Philippe Michaud.
Abstract
In this work the extraction of EPSs from culture media of Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) and membrane separation techniques has been studied. Diafiltration (DF) using a membrane with a 300 kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6 g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers.Entities:
Keywords: Diafiltration; Exopolysaccharide; Porphyridium sp.
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Year: 2012 PMID： 23313179 DOI： 10.1016/j.biortech.2012.12.038
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642