OBJECTIVE: The aim of the study is to investigate the meiotic segregation in fresh eggs from anonymous egg donors and to analyze the baseline levels of aneuploidy in this population. RESULTS: The study includes the largest series of donor eggs so far studied: 203 eggs from donors aged between 20 and 31 years. No diagnosis was obtained in 10.8 % of cases (22/ 203). The biopsy of the first and second polar bodies was completed in a sequential manner on day 0 and day 1 of embryo development. Chromosomes 13, 16, 18, 21 and 22 are analyzed by means of the FISH test. The diagnosable fertilized eggs gave an aneuploidy rate of 19.1 % (31/162), with 83.8 % (26/31) of the errors produced during meiosis I, 12.9 % (4/31) produced during meiosis II, and 3.2 % (1/31) produced during both meiosis I and II. The premature division of sister chromatids is the main source of meiotic error during Meiosis I, resulting in the creation of oocyte aneuploidy. CONCLUSIONS: FISH analysis of the first and second polar body in donor oocytes gave an aneuploidy rate of 19.1 %. This study shows the majority of errors occur during Meiosis I.
OBJECTIVE: The aim of the study is to investigate the meiotic segregation in fresh eggs from anonymous egg donors and to analyze the baseline levels of aneuploidy in this population. RESULTS: The study includes the largest series of donor eggs so far studied: 203 eggs from donors aged between 20 and 31 years. No diagnosis was obtained in 10.8 % of cases (22/ 203). The biopsy of the first and second polar bodies was completed in a sequential manner on day 0 and day 1 of embryo development. Chromosomes 13, 16, 18, 21 and 22 are analyzed by means of the FISH test. The diagnosable fertilized eggs gave an aneuploidy rate of 19.1 % (31/162), with 83.8 % (26/31) of the errors produced during meiosis I, 12.9 % (4/31) produced during meiosis II, and 3.2 % (1/31) produced during both meiosis I and II. The premature division of sister chromatids is the main source of meiotic error during Meiosis I, resulting in the creation of oocyte aneuploidy. CONCLUSIONS: FISH analysis of the first and second polar body in donor oocytes gave an aneuploidy rate of 19.1 %. This study shows the majority of errors occur during Meiosis I.
Authors: Y Verlinsky; J Cieslak; V Ivakhnenko; S Evsikov; G Wolf; M White; A Lifchez; B Kaplan; J Moise; J Valle; N Ginsberg; C Strom; A Kuliev Journal: J Assist Reprod Genet Date: 1999-04 Impact factor: 3.412
Authors: T Hassold; M Abruzzo; K Adkins; D Griffin; M Merrill; E Millie; D Saker; J Shen; M Zaragoza Journal: Environ Mol Mutagen Date: 1996 Impact factor: 3.216
Authors: C Gutiérrez-Mateo; J Benet; D Wells; P Colls; M G Bermúdez; J F Sánchez-García; J Egozcue; J Navarro; S Munné Journal: Hum Reprod Date: 2004-11-01 Impact factor: 6.918