Literature DB >> 23303494

Real-time multiplex PCR assay and melting curve analysis for identifying diarrheagenic Escherichia coli.

Tamara B Souza1, Diego M Lozer, Sônia M S Kitagawa, Liliana C Spano, Neusa P Silva, Isabel C A Scaletsky.   

Abstract

A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea.

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Year:  2013        PMID： 23303494      PMCID： PMC3592053          DOI： 10.1128/JCM.02478-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.

Authors:  T Bellin; M Pulz; A Matussek; H G Hempen; F Gunzer
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

2.  Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157.

Authors:  A W Paton; J C Paton
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

3.  WHO estimates of the causes of death in children.

Authors:  Jennifer Bryce; Cynthia Boschi-Pinto; Kenji Shibuya; Robert E Black
Journal:  Lancet       Date:  2005 Mar 26-Apr 1       Impact factor: 79.321

4.  Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp.

Authors:  K R S Aranda; U Fagundes-Neto; I C A Scaletsky
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

5.  Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells.

Authors:  S S Bilge; C R Clausen; W Lau; S L Moseley
Journal:  J Bacteriol       Date:  1989-08       Impact factor: 3.490

6.  Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection.

Authors:  S Stacy-Phipps; J J Mecca; J B Weiss
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

7.  Enteroaggregative Escherichia coli virulence factors are found to be associated with infantile diarrhea in Brazil.

Authors:  Andresa Zamboni; Sandra H Fabbricotti; Ulysses Fagundes-Neto; Isabel C A Scaletsky
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

8.  Atypical enteropathogenic Escherichia coli strains: phenotypic and genetic profiling reveals a strong association between enteroaggregative E. coli heat-stable enterotoxin and diarrhea.

Authors:  Michelle V Dulguer; Sandra H Fabbricotti; Silvia Y Bando; Carlos A Moreira-Filho; Ulysses Fagundes-Neto; Isabel C A Scaletsky
Journal:  J Infect Dis       Date:  2003-11-14       Impact factor: 5.226

9.  Detection of Shigellae and enteroinvasive Escherichia coli by amplification of the invasion plasmid antigen H DNA sequence in patients with dysentery.

Authors:  O Sethabutr; M Venkatesan; G S Murphy; B Eampokalap; C W Hoge; P Echeverria
Journal:  J Infect Dis       Date:  1993-02       Impact factor: 5.226

10.  A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital.

Authors:  Christoph Hardegen; Sabine Messler; Birgit Henrich; Klaus Pfeffer; Jens Würthner; Colin R MacKenzie
Journal:  Ann Clin Microbiol Antimicrob       Date:  2010-01-22       Impact factor: 3.944
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  4 in total

1.  Two Multiplex Real-Time PCR Assays to Detect and Differentiate Acinetobacter baumannii and Non- baumannii Acinetobacter spp. Carrying blaNDM, blaOXA-23-Like, blaOXA-40-Like, blaOXA-51-Like, and blaOXA-58-Like Genes.

Authors:  Qiu Yang; Yongyu Rui
Journal:  PLoS One       Date:  2016-07-08       Impact factor: 3.240

2.  A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses.

Authors:  Zhenzhou Wan; Ya'nan Zhang; Zhixiang He; Jia Liu; Ke Lan; Yihong Hu; Chiyu Zhang
Journal:  Int J Mol Sci       Date:  2016-11-23       Impact factor: 5.923

3.  Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR.

Authors:  Weilan Wang; Ruurd T Zijlstra; Michael G Gänzle
Journal:  BMC Microbiol       Date:  2017-05-15       Impact factor: 3.605

4.  Simultaneous Identification of Ten Bacterial Pathogens Using the Multiplex Ligation Reaction Based on the Probe Melting Curve Analysis.

Authors:  Yixiang Jiang; Lianhua He; Pingfang Wu; Xiaolu Shi; Min Jiang; Yinghui Li; Yiman Lin; Yaqun Qiu; Fang Bai; Yiqun Liao; Qingge Li; RenLi Zhang; Qinghua Hu
Journal:  Sci Rep       Date:  2017-07-19       Impact factor: 4.379
  4 in total

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