PURPOSE: To demonstrate and validate a late gadolinium enhancement (LGE) imaging protocol, optimized for tissue viability assessment in interventional mouse models of myocardial infarction. MATERIALS AND METHODS: The method uses an efficient sampling scheme using multiple slices in a single heartbeat interleaving slice packages between alternate TRs. Sampling multiple slices instead of multiple echoes or multiple k-lines achieves higher SNR efficiency, and images covering the whole heart can be obtained in 3 min. Saturation effects are exploited for an optimum compromise between contrast and speed. RESULTS: The images obtained show high hyperenhancement with good contrast-to-noise. It is shown that inversion time optimization can be reliably omitted with this scheme, and that measured infarct sizes correlate well with histological measures. CONCLUSION: Our protocol offers a new efficient tool for the measurement of infarct size in mouse models of heart disease.
PURPOSE: To demonstrate and validate a late gadolinium enhancement (LGE) imaging protocol, optimized for tissue viability assessment in interventional mouse models of myocardial infarction. MATERIALS AND METHODS: The method uses an efficient sampling scheme using multiple slices in a single heartbeat interleaving slice packages between alternate TRs. Sampling multiple slices instead of multiple echoes or multiple k-lines achieves higher SNR efficiency, and images covering the whole heart can be obtained in 3 min. Saturation effects are exploited for an optimum compromise between contrast and speed. RESULTS: The images obtained show high hyperenhancement with good contrast-to-noise. It is shown that inversion time optimization can be reliably omitted with this scheme, and that measured infarct sizes correlate well with histological measures. CONCLUSION: Our protocol offers a new efficient tool for the measurement of infarct size in mouse models of heart disease.
Authors: Guido Buonincontri; Carmen Methner; Thomas Krieg; Robert C Hawkes; T Adrian Carpenter; Stephen J Sawiak Journal: Nucl Instrum Methods Phys Res A Date: 2014-01-11 Impact factor: 1.455
Authors: Carmen Methner; Guido Buonincontri; Chou-Hui Hu; Ana Vujic; Axel Kretschmer; Stephen Sawiak; Adrian Carpenter; Johannes-Peter Stasch; Thomas Krieg Journal: PLoS One Date: 2013-12-31 Impact factor: 3.240
Authors: Eleanor Evans; Guido Buonincontri; David Izquierdo; Carmen Methner; Rob C Hawkes; Richard E Ansorge; Thomas Krieg; T Adrian Carpenter; Stephen J Sawiak Journal: IEEE Trans Nucl Sci Date: 2015-06-01 Impact factor: 1.679
Authors: Guido Buonincontri; Carmen Methner; T Adrian Carpenter; Robert C Hawkes; Stephen J Sawiak; Thomas Krieg Journal: J Vis Exp Date: 2013-12-19 Impact factor: 1.355
Authors: Guido Buonincontri; Nigel I Wood; Simon G Puttick; Alex O Ward; T Adrian Carpenter; Stephen J Sawiak; A Jennifer Morton Journal: J Huntingtons Dis Date: 2014
Authors: Edward T Chouchani; Carmen Methner; Guido Buonincontri; Chou-Hui Hu; Angela Logan; Stephen J Sawiak; Michael P Murphy; Thomas Krieg Journal: PLoS One Date: 2014-04-04 Impact factor: 3.240
Authors: Carmen Methner; Edward T Chouchani; Guido Buonincontri; Victoria R Pell; Stephen J Sawiak; Michael P Murphy; Thomas Krieg Journal: Eur J Heart Fail Date: 2014-05-31 Impact factor: 15.534