Literature DB >> 2327971

Purification and characterization of dichloromuconate cycloisomerase from Alcaligenes eutrophus JMP 134.

A E Kuhm1, M Schlömann, H J Knackmuss, D H Pieper.   

Abstract

Dichloromuconate cycloisomerase from Alcaligenes eutrophus JMP 134 was purified to homogeneity. The enzyme has an Mr of about 270,000 as determined by gel filtration and consists of six to eight subunits of identical Mr 40,000 as determined by SDS/PAGE. Mn2+ ions as well as thiol groups are required for activity. A high Km value of about 4 mM for cis,cis-muconate explains the reported low activity with this compound. Relatively high Km values were also calculated for monochloro-substituted cis,cis-muconates (300-500 microM), in contrast with the low Km value of 20 microM for 2,4-dichloro-cis,cis-muconate. The catalytic constant of the pure enzyme was 3820 min-1 when measured with 2,4-dichloro-cis,cis-muconate.

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Year:  1990        PMID： 2327971      PMCID： PMC1131220     

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  28 in total

1.  Molecular properties of cis,cis-muconate cycloisomerase from Pseudomonas putida.

Authors:  G Avigad; S Englard; B R Olsen; C Wolfenstein-Todel; R Wiggins
Journal:  J Mol Biol       Date:  1974-11-15       Impact factor: 5.469

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Metabolism of 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate by a pseudomonad.

Authors:  J Hartmann; W Reineke; H J Knackmuss
Journal:  Appl Environ Microbiol       Date:  1979-03       Impact factor: 4.792

4.  Microbial metabolism of haloaromatics: isolation and properties of a chlorobenzene-degrading bacterium.

Authors:  W Reineke; H J Knackmuss
Journal:  Appl Environ Microbiol       Date:  1984-02       Impact factor: 4.792

5.  Utilization and cooxidation of chlorinated phenols by Pseudomonas sp. B 13.

Authors:  H J Knackmuss; M Hellwig
Journal:  Arch Microbiol       Date:  1978-04-27       Impact factor: 2.552

6.  Enzymes of the beta-ketoadipate pathway in Pseudomonas putida: kinetic and magnetic resonance studies of the cis,cis-muconate cycloisomerase catalyzed reaction.

Authors:  K L Ngai; L N Ornston; R G Kallen
Journal:  Biochemistry       Date:  1983-10-25       Impact factor: 3.162

7.  Degradation of 1,4-dichlorobenzene by a Pseudomonas sp.

Authors:  J C Spain; S F Nishino
Journal:  Appl Environ Microbiol       Date:  1987-05       Impact factor: 4.792

8.  Nucleotide homology and organization of chlorocatechol oxidation genes of plasmids pJP4 and pAC27.

Authors:  D Ghosal; I S You
Journal:  Mol Gen Genet       Date:  1988-01

9.  Chemical structure and biodegradability of halogenated aromatic compounds. Halogenated muconic acids as intermediates.

Authors:  E Schmidt; G Remberg; H J Knackmuss
Journal:  Biochem J       Date:  1980-10-15       Impact factor: 3.857

10.  Bacterial metabolism of 2,4-dichlorophenoxyacetate.

Authors:  W C Evans; B S Smith; H N Fernley; J I Davies
Journal:  Biochem J       Date:  1971-05       Impact factor: 3.857
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  36 in total

1.  Two chlorocatechol catabolic gene modules on plasmid pJP4.

Authors:  Michael Schlömann
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

Review 2.  Molecular mechanisms of genetic adaptation to xenobiotic compounds.

Authors:  J R van der Meer; W M de Vos; S Harayama; A J Zehnder
Journal:  Microbiol Rev       Date:  1992-12

3.  Mechanism of chloride elimination from 3-chloro- and 2,4-dichloro-cis,cis-muconate: new insight obtained from analysis of muconate cycloisomerase variant CatB-K169A.

Authors:  U Kaulmann; S R Kaschabek; M Schlömann
Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

4.  Substrate specificity of and product formation by muconate cycloisomerases: an analysis of wild-type enzymes and engineered variants.

Authors:  M D Vollmer; H Hoier; H J Hecht; U Schell; J Gröning; A Goldman; M Schlömann
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

5.  Conversion of 2-chloro-cis,cis-muconate and its metabolites 2-chloro- and 5-chloromuconolactone by chloromuconate cycloisomerases of pJP4 and pAC27.

Authors:  M D Vollmer; M Schlömann
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

6.  Characterization of muconate and chloromuconate cycloisomerase from Rhodococcus erythropolis 1CP: indications for functionally convergent evolution among bacterial cycloisomerases.

Authors:  I P Solyanikova; O V Maltseva; M D Vollmer; L A Golovleva; M Schlömann
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

7.  Degradation of chloroaromatics: purification and characterization of maleylacetate reductase from Pseudomonas sp. strain B13.

Authors:  S R Kaschabek; W Reineke
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

8.  Formation of Dimethylmuconolactones from Dimethylphenols by Alcaligenes eutrophus JMP 134.

Authors:  D H Pieper; K Stadler-Fritzsche; H Knackmuss; K N Timmis
Journal:  Appl Environ Microbiol       Date:  1995-06       Impact factor: 4.792

9.  Importance of different tfd genes for degradation of chloroaromatics by Ralstonia eutropha JMP134.

Authors:  Iris Plumeier; Danilo Pérez-Pantoja; Sabina Heim; Bernardo González; Dietmar H Pieper
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

10.  Metabolism of 2-chloro-4-methylphenoxyacetate by Alcaligenes eutrophus JMP 134.

Authors:  D H Pieper; K Stadler-Fritzsche; K H Engesser; H J Knackmuss
Journal:  Arch Microbiol       Date:  1993       Impact factor: 2.552
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