Highly sensitive protein molecularly imprinted electro-chemical sensor based on gold microdendrites electrode and prussian blue mediated amplification.

In this paper, a novel strategy of protein molecularly-imprinted electrochemical sensor was proposed to determine Bovine Hemoglobin selectively. Gold microdendrites and prussian blue particles were electrodeposited on the surface of glassy carbon electrode respectively to improve the sensitivity of MIECS. The molecularly imprinted polymers film is synthesized by electrochemically induced redox polymerization of acrylamide in the presence of template protein. The surface feature of the modified electrode was characterized by cyclic voltammetry. The reduced electrochemical intensity which was produced by prussian blue is related to the concentration of Bovine Hemoglobin. The times of washing and incubation of modified electrode were investigated and optimized. The MIECS with improved conductivity and electrochemical performances showed good response in the range of low concentrations from 0.1 to 1.0×10³ μg/mL and the detection limit (LOD, 3σ, RSD≤4.31 %) is 0.05 μg/mL. The result demonstrates significant specific recognition toward the template protein via selective test and a reasonable regeneration in five recyclings. The proposed sensor exhibited high sensitivity and selectivity, acceptable reproducibility, and could be extended to recognize other protein.