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Literature DB >> 2325625

Expression of the beta-glucuronidase gene under the control of the CaMV 35s promoter in Schizosaccharomyces pombe.

N Pobjecky¹, G H Rosenberg, G Dinter-Gottlieb, N F Käufer.

Abstract

We have transformed Schizosaccharomyces pombe with the beta-glucuronidase (GUS) gene from Escherichia coli under the control of the plant cauliflower mosaic virus (CaMV) 35S promoter element. Efficient expression of GUS enzyme was observed. Moreover, transcription initiated at a unique site identical to that used in plant cells.

Entities: Species

Mesh：

Substances：
Glucuronidase

Year: 1990 PMID： 2325625 DOI： 10.1007/bf00260500

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

21 in total

1. Double-stranded sequencing, using mini-prep plasmids, in eleven hours.

Authors: K B Gaterman; G H Rosenberg; N F Käufer
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2. Functional regions of the cauliflower mosaic virus 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity.

Authors: D W Ow; J D Jacobs; S H Howell
Journal: Proc Natl Acad Sci U S A Date: 1987-07 Impact factor: 11.205

3. beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

Authors: R A Jefferson; S M Burgess; D Hirsh
Journal: Proc Natl Acad Sci U S A Date: 1986-11 Impact factor: 11.205

4. Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker.

Authors: C Grimm; J Kohli; J Murray; K Maundrell
Journal: Mol Gen Genet Date: 1988-12

5. Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.

Authors: M G Lee; P Nurse
Journal: Nature Date: 1987 May 7-13 Impact factor: 49.962

Review 6. Schizosaccharomyces pombe and Saccharomyces cerevisiae: a look at yeasts divided.

Authors: P Russell; P Nurse
Journal: Cell Date: 1986-06-20 Impact factor: 41.582

7. Properties of an isolated transcription stimulating sequence derived from the cauliflower mosaic virus 35S promoter.

Authors: J T Odell; S Knowlton; W Lin; C J Mauvais
Journal: Plant Mol Biol Date: 1988-05 Impact factor: 4.076

8. Transcription and expression of zein sequences in yeast under natural plant or yeast promoters.

Authors: I Coraggio; C Compagno; E Martegani; B M Ranzi; E Sala; L Alberghina; A Viotti
Journal: EMBO J Date: 1986-03 Impact factor: 11.598

9. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors: R A Jefferson; T A Kavanagh; M W Bevan
Journal: EMBO J Date: 1987-12-20 Impact factor: 11.598

10. Transcription from maize storage protein gene promoters in yeast.

Authors: P Langridge; H Eibel; J W Brown; G Feix
Journal: EMBO J Date: 1984-11 Impact factor: 11.598

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4. Novel recombinant binary vectors harbouring Basta (bar) gene as a plant selectable marker for genetic transformation of plants.

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5. Evolutionary conservation of transcriptional machinery between yeast and plants as shown by the efficient expression from the CaMV 35S promoter and 35S terminator.

Authors: H Hirt; M Kögl; T Murbacher; E Heberle-Bors
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6. Cauliflower mosaic virus P35S promoter activity in Escherichia coli.

Authors: F F Assaad; E R Signer
Journal: Mol Gen Genet Date: 1990-09

7. The GUS gene fusion system (Escherichia coli beta-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum.

Authors: Y Couteaudier; M J Daboussi; A Eparvier; T Langin; J Orcival
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