Literature DB >> 30192519

Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS.

Yuchao Chen1, Pan Mao1, Daojing Wang1.   

Abstract

Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA).

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Year:  2018        PMID： 30192519      PMCID： PMC6143396          DOI： 10.1021/acs.analchem.8b02699

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  17 in total

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Authors:  Julia Maria Burkhart; Cornelia Schumbrutzki; Stefanie Wortelkamp; Albert Sickmann; René Peiman Zahedi
Journal:  J Proteomics       Date:  2011-11-30       Impact factor: 4.044

2.  LC-MS/MS approach for quantification of therapeutic proteins in plasma using a protein internal standard and 2D-solid-phase extraction cleanup.

Authors:  Ziping Yang; Michael Hayes; Xinping Fang; Michael P Daley; Seth Ettenberg; Francis L S Tse
Journal:  Anal Chem       Date:  2007-11-14       Impact factor: 6.986

3.  An automated and multiplexed method for high throughput peptide immunoaffinity enrichment and multiple reaction monitoring mass spectrometry-based quantification of protein biomarkers.

Authors:  Jeffrey R Whiteaker; Lei Zhao; Leigh Anderson; Amanda G Paulovich
Journal:  Mol Cell Proteomics       Date:  2009-10-20       Impact factor: 5.911

Review 4.  Quality control in mass spectrometry-based proteomics.

Authors:  Wout Bittremieux; David L Tabb; Francis Impens; An Staes; Evy Timmerman; Lennart Martens; Kris Laukens
Journal:  Mass Spectrom Rev       Date:  2017-09-07       Impact factor: 10.946

5.  Investigation of Ion Transmission Effects on Intact Protein Quantification in a Triple Quadrupole Mass Spectrometer.

Authors:  Evelyn H Wang; Dananjaya Kalu Appulage; Erin A McAllister; Kevin A Schug
Journal:  J Am Soc Mass Spectrom       Date:  2017-05-30       Impact factor: 3.109

6.  Parallel reaction monitoring for high resolution and high mass accuracy quantitative, targeted proteomics.

Authors:  Amelia C Peterson; Jason D Russell; Derek J Bailey; Michael S Westphall; Joshua J Coon
Journal:  Mol Cell Proteomics       Date:  2012-08-03       Impact factor: 5.911

7.  Evaluation of intact mass spectrometry for the quantitative analysis of protein therapeutics.

Authors:  Ashley C Gucinski; Michael T Boyne
Journal:  Anal Chem       Date:  2012-09-04       Impact factor: 6.986

8.  Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA).

Authors:  Rudolf M Lequin
Journal:  Clin Chem       Date:  2005-09-22       Impact factor: 8.327

9.  Personal omics profiling reveals dynamic molecular and medical phenotypes.

Authors:  Rui Chen; George I Mias; Jennifer Li-Pook-Than; Lihua Jiang; Hugo Y K Lam; Rong Chen; Elana Miriami; Konrad J Karczewski; Manoj Hariharan; Frederick E Dewey; Yong Cheng; Michael J Clark; Hogune Im; Lukas Habegger; Suganthi Balasubramanian; Maeve O'Huallachain; Joel T Dudley; Sara Hillenmeyer; Rajini Haraksingh; Donald Sharon; Ghia Euskirchen; Phil Lacroute; Keith Bettinger; Alan P Boyle; Maya Kasowski; Fabian Grubert; Scott Seki; Marco Garcia; Michelle Whirl-Carrillo; Mercedes Gallardo; Maria A Blasco; Peter L Greenberg; Phyllis Snyder; Teri E Klein; Russ B Altman; Atul J Butte; Euan A Ashley; Mark Gerstein; Kari C Nadeau; Hua Tang; Michael Snyder
Journal:  Cell       Date:  2012-03-16       Impact factor: 41.582

10.  Plug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry.

Authors:  Robert T Lawrence; Brian C Searle; Ariadna Llovet; Judit Villén
Journal:  Nat Methods       Date:  2016-03-28       Impact factor: 28.547
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  2 in total

1.  A Rapid LC-MS/MS-PRM Assay for Serologic Quantification of Sialylated O-HPX Glycoforms in Patients with Liver Fibrosis.

Authors:  Aswini Panigrahi; Julius Benicky; Renhuizi Wei; Jaeil Ahn; Radoslav Goldman; Miloslav Sanda
Journal:  Molecules       Date:  2022-03-29       Impact factor: 4.411

2.  Multiplex Mass Spectrometry Analysis of Amyloid Proteins in Human Plasma for Alzheimer's Disease Diagnosis.

Authors:  Weimin Ni; William Jagust; Daojing Wang
Journal:  J Proteome Res       Date:  2021-07-27       Impact factor: 5.370
  2 in total

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