Literature DB >> 23236063

A novel bacterium-free method for generation of flavivirus infectious DNA by circular polymerase extension reaction allows accurate recapitulation of viral heterogeneity.

Judith Edmonds1, Erinke van Grinsven, Natalie Prow, Angela Bosco-Lauth, Aaron C Brault, Richard A Bowen, Roy A Hall, Alexander A Khromykh.   

Abstract

A novel bacterium-free approach for rapid assembly of flavivirus infectious cDNAs using circular polymerase extension reaction was applied to generate infectious cDNA for the virulent New South Wales isolate of the Kunjin strain of West Nile virus (KUNV) that recently emerged in Australia. Recovered virus recapitulated the genetic heterogeneity present in the original isolate. The approach was utilized to generate viral mutants with designed phenotypic properties and to identify E protein glycosylation as one of the virulence determinants.

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Year:  2012        PMID： 23236063      PMCID： PMC3571472          DOI： 10.1128/JVI.03162-12

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  26 in total

1.  A new strategy in design of +RNA virus infectious clones enabling their stable propagation in E. coli.

Authors:  V Yamshchikov; V Mishin; F Cominelli
Journal:  Virology       Date:  2001-03-15       Impact factor: 3.616

2.  Biological significance of glycosylation of the envelope protein of Kunjin virus.

Authors:  J H Scherret; J S Mackenzie; A A Khromykh; R A Hall
Journal:  Ann N Y Acad Sci       Date:  2001-12       Impact factor: 5.691

3.  Amplification and cloning of the full-length genome of Japanese encephalitis virus by a novel long RT-PCR protocol in a cosmid vector.

Authors:  F Zhang; Q Huang; W Ma; S Jiang; Y Fan; H Zhang
Journal:  J Virol Methods       Date:  2001-08       Impact factor: 2.014

4.  DNA vaccine coding for the full-length infectious Kunjin virus RNA protects mice against the New York strain of West Nile virus.

Authors:  Roy A Hall; Debra J Nisbet; Kim B Pham; Alyssa T Pyke; Greg A Smith; Alexander A Khromykh
Journal:  Proc Natl Acad Sci U S A       Date:  2003-08-13       Impact factor: 11.205

5.  Monoclonal antibodies to Kunjin and Kokobera viruses.

Authors:  R A Hall; G W Burgess; B H Kay; P Clancy
Journal:  Immunol Cell Biol       Date:  1991-02       Impact factor: 5.126

6.  Infectious RNA transcribed from stably cloned full-length cDNA of dengue type 4 virus.

Authors:  C J Lai; B T Zhao; H Hori; M Bray
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

7.  Epitope analysis of the envelope and non-structural glycoproteins of Murray Valley encephalitis virus.

Authors:  R A Hall; B H Kay; G W Burgess; P Clancy; I D Fanning
Journal:  J Gen Virol       Date:  1990-12       Impact factor: 3.891

8.  Infectious cDNA clone of the epidemic west nile virus from New York City.

Authors:  Pei-Yong Shi; Mark Tilgner; Michael K Lo; Kim A Kent; Kristen A Bernard
Journal:  J Virol       Date:  2002-06       Impact factor: 5.103

9.  Development and application of a reverse genetics system for Japanese encephalitis virus.

Authors:  Sang-Im Yun; Seok-Yong Kim; Charles M Rice; Young-Min Lee
Journal:  J Virol       Date:  2003-06       Impact factor: 5.103

10.  A stable full-length yellow fever virus cDNA clone and the role of conserved RNA elements in flavivirus replication.

Authors:  Peter J Bredenbeek; Engbert A Kooi; Brett Lindenbach; Nicolette Huijkman; Charles M Rice; Willy J M Spaan
Journal:  J Gen Virol       Date:  2003-05       Impact factor: 3.891
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  32 in total

1.  In Vivo Dynamics of Reporter Flaviviridae Viruses.

Authors:  Tomokazu Tamura; Manabu Igarashi; Bazarragchaa Enkhbold; Tatsuya Suzuki; Masatoshi Okamatsu; Chikako Ono; Hiroyuki Mori; Takuma Izumi; Asuka Sato; Yuzy Fauzyah; Toru Okamoto; Yoshihiro Sakoda; Takasuke Fukuhara; Yoshiharu Matsuura
Journal:  J Virol       Date:  2019-10-29       Impact factor: 5.103

2.  Recovery and Genetic Characterization of a West Nile Virus Isolate from China.

Authors:  Yan Guo; Hongjiang Wang; Songtao Xu; Hangyu Zhou; Chao Zhou; Shihong Fu; Mengli Cheng; Fan Li; Yongqiang Deng; Xiaofeng Li; Huanyu Wang; Cheng-Feng Qin
Journal:  Virol Sin       Date:  2020-07-06       Impact factor: 4.327

3.  Characterization of Recombinant Flaviviridae Viruses Possessing a Small Reporter Tag.

Authors:  Tomokazu Tamura; Takasuke Fukuhara; Takuro Uchida; Chikako Ono; Hiroyuki Mori; Asuka Sato; Yuzy Fauzyah; Toru Okamoto; Takeshi Kurosu; Yin Xiang Setoh; Michio Imamura; Norbert Tautz; Yoshihiro Sakoda; Alexander A Khromykh; Kazuaki Chayama; Yoshiharu Matsuura
Journal:  J Virol       Date:  2018-01-02       Impact factor: 5.103

4.  Characterization of cis-Acting RNA Elements of Zika Virus by Using a Self-Splicing Ribozyme-Dependent Infectious Clone.

Authors:  Zhong-Yu Liu; Jiu-Yang Yu; Xing-Yao Huang; Hang Fan; Xiao-Feng Li; Yong-Qiang Deng; Xue Ji; Meng-Li Cheng; Qing Ye; Hui Zhao; Jian-Feng Han; Xiao-Ping An; Tao Jiang; Bo Zhang; Yi-Gang Tong; Cheng-Feng Qin
Journal:  J Virol       Date:  2017-10-13       Impact factor: 5.103

5.  An Infectious cDNA Clone of Zika Virus to Study Viral Virulence, Mosquito Transmission, and Antiviral Inhibitors.

Authors:  Chao Shan; Xuping Xie; Antonio E Muruato; Shannan L Rossi; Christopher M Roundy; Sasha R Azar; Yujiao Yang; Robert B Tesh; Nigel Bourne; Alan D Barrett; Nikos Vasilakis; Scott C Weaver; Pei-Yong Shi
Journal:  Cell Host Microbe       Date:  2016-05-16       Impact factor: 21.023

6.  Novel Synthesis and Phenotypic Analysis of Mutant Clouds for Hepatitis E Virus Genotype 1.

Authors:  Shubhra Agarwal; Prasith Baccam; Rakesh Aggarwal; Naga Suresh Veerapu
Journal:  J Virol       Date:  2018-01-30       Impact factor: 5.103

7.  A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses.

Authors:  Alberto A Amarilla; Julian D J Sng; Rhys Parry; Joshua M Deerain; James R Potter; Yin Xiang Setoh; Daniel J Rawle; Thuy T Le; Naphak Modhiran; Xiaohui Wang; Nias Y G Peng; Francisco J Torres; Alyssa Pyke; Jessica J Harrison; Morgan E Freney; Benjamin Liang; Christopher L D McMillan; Stacey T M Cheung; Darwin J Da Costa Guevara; Joshua M Hardy; Mark Bettington; David A Muller; Fasséli Coulibaly; Frederick Moore; Roy A Hall; Paul R Young; Jason M Mackenzie; Jody Hobson-Peters; Andreas Suhrbier; Daniel Watterson; Alexander A Khromykh
Journal:  Nat Commun       Date:  2021-06-08       Impact factor: 14.919

8.  "ISA-Lation" of Single-Stranded Positive-Sense RNA Viruses from Non-Infectious Clinical/Animal Samples.

Authors:  Fabien Aubry; Antoine Nougairède; Lauriane de Fabritus; Géraldine Piorkowski; Ernest A Gould; Xavier de Lamballerie
Journal:  PLoS One       Date:  2015-09-25       Impact factor: 3.240

Review 9.  Applications of Deep Mutational Scanning in Virology.

Authors:  Thomas D Burton; Nicholas S Eyre
Journal:  Viruses       Date:  2021-05-28       Impact factor: 5.048

10.  The relationship between the structure of the tick-borne encephalitis virus strains and their pathogenic properties.

Authors:  Sergei I Belikov; Ilya G Kondratov; Ulyana V Potapova; Galina N Leonova
Journal:  PLoS One       Date:  2014-04-16       Impact factor: 3.240
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