| Literature DB >> 23224160 |
Claire Robertson1, Steven C George.
Abstract
Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction, noise, and image morphology were examined analytically and their effects on ICS analysis modeled. A series of recommendations was built based on this analysis.Mesh:
Year: 2012 PMID: 23224160 PMCID: PMC3414238 DOI: 10.1117/1.JBO.17.8.080801
Source DB: PubMed Journal: J Biomed Opt ISSN: 1083-3668 Impact factor: 3.170