Literature DB >> 18089858

Image correlation spectroscopy.

Anja Nohe1, Nils O Petersen.   

Abstract

Membrane domains, such as caveolae and clathrin-coated pits, regulate cell signaling and protein internalization in the plasma membrane. Fluorescence imaging and microscopy provide an opportunity to determine the receptor protein dynamics of membrane microdomains. The family of image correlation spectroscopy (ICS) techniques provides powerful tools with which to measure the aggregation, clustering, and dynamics of proteins in the plasma membrane. ICS is used to calculate the cluster density and the degree of aggregation of plasma membrane proteins, whereas image cross-correlation spectroscopy (ICCS) measures the fraction of colocalization of two proteins. Dynamic image correlation spectroscopy (DICS) can be used to analyze protein dynamics on the cell surface during live-cell imaging.

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Year:  2007        PMID: 18089858     DOI: 10.1126/stke.4172007pl7

Source DB:  PubMed          Journal:  Sci STKE        ISSN: 1525-8882


  15 in total

1.  Casein kinase 2 beta-subunit is a regulator of bone morphogenetic protein 2 signaling.

Authors:  Beth Bragdon; Shayamala Thinakaran; Oleksandra Moseychuk; Daniel King; Kira Young; David W Litchfield; Nils O Petersen; Anja Nohe
Journal:  Biophys J       Date:  2010-08-04       Impact factor: 4.033

2.  Quantitative determination of spatial protein-protein correlations in fluorescence confocal microscopy.

Authors:  Yong Wu; Mansoureh Eghbali; Jimmy Ou; Rong Lu; Ligia Toro; Enrico Stefani
Journal:  Biophys J       Date:  2010-02-03       Impact factor: 4.033

3.  Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy.

Authors:  Adam C Parslow; Andrew H A Clayton; Peter Lock; Andrew M Scott
Journal:  J Vis Exp       Date:  2018-08-02       Impact factor: 1.355

4.  Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.

Authors:  Claire Robertson; Steven C George
Journal:  J Biomed Opt       Date:  2012-08       Impact factor: 3.170

5.  Altered plasma membrane dynamics of bone morphogenetic protein receptor type Ia in a low bone mass mouse model.

Authors:  Beth Bragdon; Alex D'Angelo; Lauren Gurski; Jeremy Bonor; Kathryn L Schultz; Wesley G Beamer; Clifford J Rosen; Anja Nohe
Journal:  Bone       Date:  2011-10-22       Impact factor: 4.398

Review 6.  Quantifying translational mobility in neurons: comparison between current optical techniques.

Authors:  Sally A Kim; Hugo Sanabria; Michelle A Digman; Enrico Gratton; Petra Schwille; Warren R Zipfel; M Neal Waxham
Journal:  J Neurosci       Date:  2010-12-08       Impact factor: 6.167

7.  Bone morphogenetic protein receptor type Ia localization causes increased BMP2 signaling in mice exhibiting increased peak bone mass phenotype.

Authors:  Beth Bragdon; Jeremy Bonor; Kathryn L Shultz; Wesley G Beamer; Clifford J Rosen; Anja Nohe
Journal:  J Cell Physiol       Date:  2012-07       Impact factor: 6.384

8.  Inhibition of CK2 binding to BMPRIa induces C2C12 differentiation into osteoblasts and adipocytes.

Authors:  Oleksandra Moseychuk; Hemanth Akkiraju; Joyita Dutta; Alex D'Angelo; Beth Bragdon; Randall L Duncan; Anja Nohe
Journal:  J Cell Commun Signal       Date:  2013-05-01       Impact factor: 5.782

9.  CK2.1, a novel peptide, induces articular cartilage formation in vivo.

Authors:  Hemanth Akkiraju; Jeremy Bonor; Anja Nohe
Journal:  J Orthop Res       Date:  2016-07-08       Impact factor: 3.494

10.  FRET reveals novel protein-receptor interaction of bone morphogenetic proteins receptors and adaptor protein 2 at the cell surface.

Authors:  Beth Bragdon; Shayamala Thinakaran; Jeremy Bonor; T Michael Underhill; Nils O Petersen; Anja Nohe
Journal:  Biophys J       Date:  2009-09-02       Impact factor: 4.033

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